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Serum Response Factor SRF Antibody (2G2)

Images

 
Immunocytochemistry/ Immunofluorescence: Serum Response Factor SRF Antibody (2G2) [H00006722-M01] - Analysis of monoclonal antibody to SRF on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Serum Response Factor SRF Antibody (2G2) [H00006722-M01] - Detection limit for recombinant GST tagged SRF is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Serum Response Factor SRF Antibody (2G2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SRF (AAH48211, 406 a.a. ~ 508 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE
Specificity
SRF - serum response factor (c-fos serum response element-binding transcription factor)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SRF
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Serum Response Factor SRF Antibody (2G2)

  • MCM1
  • serum response factor (c-fos serum response element-binding transcriptionfactor)
  • Serum Response Factor SRF
  • serum response factor
  • SRF

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRF
Entrez
OMIM
Uniprot