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SDHA Antibody

Images

 
Independent Antibodies: Western Blot: SDHA Antibody [NBP2-56988] - Analysis using Anti-SDHA antibody NBP2-56988 (A) shows similar pattern to independent antibody NBP2-32594 (B).
Immunocytochemistry/ Immunofluorescence: SDHA Antibody [NBP2-56988] - Staining of human cell line SiHa shows localization to nucleoli & mitochondria. Antibody staining is shown in green.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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SDHA Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEES
Predicted Species
Mouse (94%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHA
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SDHA Recombinant Protein Antigen (NBP2-56988PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SDHA Antibody

  • CMD1GG
  • flavoprotein subunit of complex II
  • FP
  • MC2DN1
  • NDAXOA
  • PGL5
  • PPGL5
  • SDH1
  • SDH2
  • SDHA
  • SDHF
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHA Antibody (NBP2-56988) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SDHA Products

Research Areas for SDHA Antibody (NBP2-56988)

Find related products by research area.

Blogs on SDHA.

SDHA - oxidative enzyme in the citric acid cycle
Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re...  Read full blog post.

SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism
Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons ...  Read full blog post.

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Bioinformatics

Gene Symbol SDHA