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RIPK1/RIP1 Antibody

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Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: RIPK1/RIP1 Antibody [NBP2-20166] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RIPK1/RIP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RIP. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for RIPK1/RIP1 Antibody

  • Cell death protein RIP
  • EC 2.7.11.1
  • receptor (TNFRSF)-interacting serine-threonine kinase 1
  • receptor interacting protein
  • Receptor-interacting protein 1
  • receptor-interacting serine/threonine-protein kinase 1
  • RIP
  • Rip1 kinase
  • RIP1
  • RIP-1
  • RIPFLJ39204
  • RIPK1
  • Serine/threonine-protein kinase RIP

Background

Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol RIPK1
Uniprot