Home » IFN-alpha » IFN-alpha Proteins and Enzymes » Recombinant Porcine IFN-alpha (Mammalian) Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Porcine IFN-alpha (Mammalian) Protein

 (7 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for IFN-alpha (17105-1).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity PoSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Porcine IFN-alpha (Mammalian) Protein Summary

Details of Functionality

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37 (2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

The units are determined by use of a Human IFN Alpha A (Hu IFN-alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu IFN-alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257(1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Source

cDNA obtained from porcine leukocyte mRNA expressed in mammalian cells.
Accession # NM_214393.1
Accession #
Protein/Peptide Type
Recombinant Proteins
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
17105-1 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Phosphate buffered saline with 0.1% bovine serum albumin (BSA)

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine IFN-alpha (Mammalian) Protein

  • IFNA
  • IFNA2
  • IFNA2b
  • IFNalpha
  • IFN-alpha
  • IFN-alphaA
  • INFA2
  • interferon alpha 2
  • LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Customers Who Viewed This Item Also Viewed...

IFN-alpha 1
Antibody
NBP2-75930
Western Blot IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFN-beta
[Unconjugated]
8499-IF
Binding Activity IFN-beta [Unconjugated]
(49 Publications)
Species: Hu
Applications: BA
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(370 Publications)
Species: Hu
Applications: BA
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IRF7 Antibody
(SC0617)
NBP2-67634
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunohistochemistry-Paraffin IRF7 Antibody (SC0617)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(446 Publications)
Species: Hu
Applications: BA
IFN-alpha 13
Antibody (3F9)
H00003447-M03
Western Blot IFN-alpha 13 Antibody (3F9)
Sandwich ELISA IFN-alpha 13 Antibody (3F9)
Species: Hu
Applications: ELISA, S-ELISA, WB
IRF3 Antibody
(SD2062)
NBP2-67741
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunocytochemistry/ Immunofluorescence IRF3 Antibody (SD2062)
Immunohistochemistry-Paraffin IRF3 Antibody (SD2062)
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
p15INK4b/CDKN2B
Antibody (OTI3B6)
NBP2-45603
Flow Cytometry p15INK4b/CDKN2B Antibody (OTI3B6)
Immunohistochemistry p15INK4b/CDKN2B Antibody (OTI3B6)
Immunohistochemistry p15INK4b/CDKN2B Antibody (OTI3B6)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
MuRF1/TRIM63
Antibody [Unconjugate
AF5366
Western Blot MuRF1/TRIM63 Antibody [Unconjugated]
Simple Western MuRF1/TRIM63 Antibody [Unconjugated]
Western Blot MuRF1/TRIM63 Antibody [Unconjugated]
(26 Publications)
Species: Hu, Mu, Rt
Applications: Simple Western, WB
IRF1 Antibody
AF4715
Western Blot IRF1 Antibody
(1 Review)
(2 Publications)
Species: Mu
Applications: WB
IRF5 Antibody
NB100-1092
Western Blot IRF5 Antibody
Western Blot IRF5 Antibody
Western Blot IRF5 Antibody
(9 Publications)
Species: Hu, Mu
Applications: ChIP, Flow, KD, PEP-ELISA, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(808 Publications)
Species: Hu
Applications: BA
GST Epitope Tag
Antibody - BSA Free
NB600-326
Western Blot GST Epitope Tag Antibody - BSA Free
Simple Western GST Epitope Tag Antibody - BSA Free
Simple Western GST Epitope Tag Antibody - BSA Free
(2 Reviews)
(18 Publications)
Species: ET
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Recombinant
Porcine IFN-alpha (Ma ...
17105-1
Recombinant Porcine IFN-alpha (Mammalian) Protein
Species: Po
Applications: Bioactivity

Publications for IFN-alpha (17105-1)(7)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(5)
ELISA (Standard)
(1)
In Vivo
(1)
All Applications
Filter By Species
Porcine
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 17105-1 Applications Species
Ba, C;Tian, C;Bo, X;Xu, C;Zhang, Y;Sun, X;Nan, Y;Wu, C; Porcine HERC6 acts as major E3 ligase for ISGylation and is auto-ISGylated for effective ISGylation in porcine Microbial pathogenesis 2024-03-29 [PMID: 38554778] (Bioassay, Porcine) Bioassay Porcine
Z Wang, J Chen, QG Zhang, K Huang, D Ma, Q Du, D Tong, Y Huang Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR Veterinary Microbiology, 2022-01-22;266(0):109354. 2022-01-22 [PMID: 35085949] (Bioassay, In Vivo, Porcine) Bioassay, In Vivo Porcine
H Liu, B Shi, Z Zhang, B Zhao, G Zhao, Y Li, Y Nan PRRSV Vaccine Strain-Induced Secretion of Extracellular ISG15 Stimulates Porcine Alveolar Macrophage Antiviral Response against PRRSV Viruses, 2020-09-10;12(9):. 2020-09-10 [PMID: 32927637] (Bioassay, Porcine) Bioassay Porcine
J Su, X Zhang, B He, X Ge, J Han, L Zhou, X Guo, H Yang Identification of three site mutations in nonstructural protein 1 β, glycoprotein 3 and glycoprotein 5 that correlate with increased interferon α resistance of porcine reproductive and respiratory syndrome virus Vet. Microbiol., 2019-08-19;236(0):108395. 2019-08-19 [PMID: 31500730] (Bioassay, Porcine) Bioassay Porcine
YL Li, L Darwich, E Mateu Characterization of the attachment and infection by Porcine reproductive and respiratory syndrome virus 1 isolates in bone marrow-derived dendritic cells Vet. Microbiol., 2018-08-11;223(0):181-188. 2018-08-11 [PMID: 30173745] (Porcine) Porcine
Sang Y, Brichalli W, Rowland R, Blecha F Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses. PLoS ONE, 2014-02-05;9(2):e87613. 2014-02-05 [PMID: 24505295] (Bioassay, Porcine) Bioassay Porcine
Miller LC, Zanella EL, Waters WR Cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus. Clin. Vaccine Immunol., 2010-03-10;17(5):728-34. 2010-03-10 [PMID: 20219878] (ELISA (Standard), Porcine) ELISA (Standard) Porcine

Reviews for IFN-alpha (17105-1) (0)

There are no reviews for IFN-alpha (17105-1). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IFN-alpha (17105-1) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IFN-alpha Products

Blogs on IFN-alpha.

Check out the latest blog posts on IFN-alpha.
STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
p53 Antibody (PAb 240) - BSA Free
NB200-103
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
IFN-gamma [Unconjugated]
285-IF-100

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Porcine IFN-alpha (Mammalian) Protein and receive a gift card or discount.


Bioinformatics

Uniprot