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Recombinant Mouse Wnt-3a Protein, CF

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Recombinant Mouse Wnt-3a (Catalog # 1324-WN/CF) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The activity is approximately 10-fold greater than the top competitor's Wnt-3a.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Wnt-3a Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Fischer, L. et al. (2002) J. Biol. Chem. 277:30870. The ED50 for this effect is ≤5 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.

Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3a protein
Ser19-Lys352
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt3a
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41 kDa, reducing conditions
Publications
Read Publications using
1324-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 40 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-3a Protein, CF

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor shares 100% aa identity with mouse Wnt-3a aa 63-352 (8), and also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13-15).
  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Entrez Accession # NP_001100475.
  9. Dunty Jr. W. C. et al. (2008) Development 135:85.
  10. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  11. Lee, S. M. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Willert, K. et al. (2003) Nature 423:6938.
  14. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  15. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

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Publications for Wnt-3a (1324-WN/CF)(192)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Rabbit, Transgenic Mouse.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, In Vivo, Organoid Culture, Receptor Binding Assay.


Filter By Application
Binding Assay
(1)
Bioassay
(161)
Cell Culture
(19)
In Vivo
(7)
Organoid Culture
(1)
Receptor Binding Assay
(1)
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Filter By Species
Human
(40)
Mouse
(144)
Rat
(6)
Chicken
(3)
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(1)
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(1)
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Showing Publications 1 - 10 of 192. Show All 192 Publications.
Publications using 1324-WN/CF Applications Species
Ortabozkoyun, H;Huang, PY;Cho, H;Tsirigos, A;Mazzoni, E;Reinberg, D; Novel Chromatin Insulating Activities Uncovered upon Eliminating Known Insulators in vivo bioRxiv : the preprint server for biology 2023-04-25 [PMID: 37162865] (Bioassay, Mouse) Bioassay Mouse
M Nomura, Y Yokoyama, D Yoshimura, Y Minagawa, A Yamamoto, Y Tanaka, N Sekiguchi, D Marukawa, M Ichihara, H Itakura, K Matsumoto, Y Morimoto, H Tomihara, A Inoue, T Ogino, N Miyoshi, H Takahashi, H Takahashi, M Uemura, S Kobayashi, T Mizushima, T Anada, M Mori, Y Doki, M Tanaka, H Eguchi, H Yamamoto Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835361] (Bioassay, Human) Bioassay Human
YF Wong, Y Kumar, M Proks, JAR Herrera, MM Rothová, RS Monteiro, S Pozzi, RE Jennings, NA Hanley, WA Bickmore, JM Brickman Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation Nature Cell Biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36690849] (Bioassay, Human) Bioassay Human
LA Dada, LC Welch, ND Magnani, Z Ren, H Han, PL Brazee, D Celli, AS Flozak, A Weng, MM Herrerias, V Kryvenko, I Vadász, CE Runyan, H Abdala-Val, M Shigemura, SM Casalino-M, AV Misharin, GRS Budinger, CJ Gottardi, JI Sznajder Hypercapnia alters stromal-derived Wnt production limiting beta-catenin signaling and proliferation in alveolar type 2 cells JCI Insight, 2023-02-22;0(0):. 2023-02-22 [PMID: 36626234] (Bioassay, Mouse) Bioassay Mouse
Y Niu, CA Ferreira A, X Li, E Kamali, O Haagen Nie, C Storgaard, M Frödin Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select Nature Genetics, 2022-12-05;54(12):1983-1993. 2022-12-05 [PMID: 36471068] (Bioassay, Human) Bioassay Human
N Pentinmikk, R Lozano, S Scharaw, S Andersson, JI Englund, D Castillo-A, A Gallagher, M Broberg, KY Song, A Sola Carva, AT Speidel, M Sundstrom, N Allbritton, MM Stevens, OD Klein, A Teixeira, P Katajisto Cellular shape reinforces niche to stem cell signaling in the small intestine Science Advances, 2022-10-14;8(41):eabm1847. 2022-10-14 [PMID: 36240269] (Bioassay, Mouse) Bioassay Mouse
H Marei, WK Tsai, YS Kee, K Ruiz, J He, C Cox, T Sun, S Penikalapa, P Dwivedi, M Choi, D Kan, P Saenz-Lope, K Dorighi, P Zhang, YT Kschonsak, N Kljavin, D Amin, I Kim, AG Mancini, T Nguyen, C Wang, E Janezic, A Doan, E Mai, H Xi, C Gu, M Heinlein, B Biehs, J Wu, I Lehoux, S Harris, L Comps-Agra, D Seshasayee, FJ de Sauvage, M Grimmer, J Li, NJ Agard, F de Sousa E Antibody targeting of E3 ubiquitin ligases for receptor degradation Nature, 2022-09-21;610(7930):182-189. 2022-09-21 [PMID: 36131013] (Bioassay, Human) Bioassay Human
G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes Journal of Cell Science, 2022-10-10;0(0):. 2022-10-10 [PMID: 36124643] (Cell Culture, Human) Cell Culture Human
Z Wang, R Fan, A Russo, FM Cernilogar, A Nuber, S Schirge, I Shcherbako, I Dzhilyanov, E Ugur, T Anton, L Richter, H Leonhardt, H Lickert, G Schotta Dominant role of DNA methylation over H3K9me3 for IAP silencing in endoderm Nature Communications, 2022-09-19;13(1):5447. 2022-09-19 [PMID: 36123357] (Bioassay, Mouse) Bioassay Mouse
G Liu, J Chen, X Wang, Y Liu, Y Ma, X Tu Functionalized 3D-Printed ST2/Gelatin Methacryloyl/Polcaprolactone Scaffolds for Enhancing Bone Regeneration with Vascularization International Journal of Molecular Sciences, 2022-07-28;23(15):. 2022-07-28 [PMID: 35955478] (Cell Culture, Mouse) Cell Culture Mouse
Show All 192 Publications.

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Bioinformatics

Gene Symbol Wnt3a
Uniprot