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Recombinant Mouse IL-1 beta/IL-1F2 Protein, CF

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Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 2‑10 pg/mL.
Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 2‑10 pg/mL.
1 μg/lane of Recombinant Mouse IL‑1 beta /IL‑1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
401-ML-005
Carrier Free
401-ML-025/CF
Carrier Free
401-ML-010/CF
Carrier Free
401-ML-100/CF
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Recombinant Mouse IL-1 beta/IL-1F2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 2-10 pg/mL.
Source
E. coli-derived mouse IL-1 beta/IL-1F2 protein
Val118-Ser269, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il1b
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
401-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 beta/IL-1F2 Protein, CF

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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Publications for IL-1 beta/IL-1F2 (401-ML/CF)(252)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria - Porphyromonas gingivalis, Transgenic Mouse.

We have publications tested in 13 applications: Binding Assay, Bioassay, Cell Culture, Degradation, Differentiation, ELISA (Standard), ELISA Standard, IHC, In Vivo, Organ Culture, Tissue Culture, Western Blot, Western Blot Control.


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Binding Assay
(1)
Bioassay
(171)
Cell Culture
(22)
Degradation
(1)
Differentiation
(2)
ELISA (Standard)
(8)
ELISA Standard
(1)
IHC
(1)
In Vivo
(47)
Organ Culture
(1)
Tissue Culture
(2)
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(2)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(241)
Rat
(3)
Bacteria - Porphyromonas gingivalis
(1)
Transgenic Mouse
(4)
All Species
Showing Publications 1 - 10 of 252. Show All 252 Publications.
Publications using 401-ML/CF Applications Species
WS Lian, RW Wu, JY Ko, YS Chen, SY Wang, H Jahr, FS Wang Inhibition of Histone Lysine Demethylase 6A Promotes Chondrocytic Activity and Attenuates Osteoarthritis Development through Repressing H3K27me3 Enhancement of Wnt10a The international journal of biochemistry & cell biology, 2023-03-05;0(0):106394. 2023-03-05 [PMID: 36871937] (Bioassay, Mouse) Bioassay Mouse
Y Liao, L Sun, M Nie, J Li, X Huang, S Heng, W Zhang, T Xia, Z Guo, Q Zhao, LJ Zhang Modulation of Skin Inflammatory Responses by Aluminum Adjuvant Pharmaceutics, 2023-02-08;15(2):. 2023-02-08 [PMID: 36839900] (Bioassay, Mouse) Bioassay Mouse
CM Schafer, S Martin-Alm, K Kurylowicz, N Dufton, L Osuna-Alma, ML Wu, CF Johnson, AV Shah, DO Haskard, A Buxton, E Willis, K Wheeler, S Turner, M Chlebicz, RP Scott, S Kovats, A Cleuren, GM Birdsey, AM Randi, CT Griffin Cytokine-Mediated Degradation of the Transcription Factor ERG Impacts the Pulmonary Vascular Response to Systemic Inflammatory Challenge bioRxiv : the preprint server for biology, 2023-02-11;0(0):. 2023-02-11 [PMID: 36798267] (Bioassay, Mouse) Bioassay Mouse
RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu Crosstalk between ILC2s and Th2 cells varies among mouse models Cell Reports, 2023-02-02;42(2):112073. 2023-02-02 [PMID: 36735533] (Bioassay, Mouse) Bioassay Mouse
PA Dumesic, SE Wilensky, S Bose, JG Van Vranke, SP Gygi, BM Spiegelman RBM43 links adipose inflammation and energy expenditure through translational regulation of PGC1alpha bioRxiv : the preprint server for biology, 2023-01-07;0(0):. 2023-01-07 [PMID: 36712038] (Bioassay, Mouse) Bioassay Mouse
IC Clark, MA Wheeler, HG Lee, Z Li, LM Sanmarco, S Thaploo, CM Polonio, SW Shin, G Scalisi, AR Henry, JM Rone, F Giovannoni, M Charabati, CF Akl, DM Aleman, SEJ Zandee, A Prat, DC Douek, EA Boritz, FJ Quintana, AR Abate Identification of astrocyte regulators by nucleic acid cytometry Nature, 2023-01-04;0(0):. 2023-01-04 [PMID: 36599367] (Bioassay, Mouse) Bioassay Mouse
G Wang, E Sweren, W Andrews, Y Li, J Chen, Y Xue, E Wier, MP Alphonse, L Luo, Y Miao, R Chen, D Zeng, S Lee, A Li, E Dare, D Kim, NK Archer, SK Reddy, L Resar, Z Hu, EA Grice, MA Kane, LA Garza Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1alpha signaling and glutamine metabolism Science Advances, 2023-01-04;9(1):eabo7555. 2023-01-04 [PMID: 36598999] (Bioassay, Human, Mouse) Bioassay Human, Mouse
CK Cheng, X Lin, Y Pu, JKY Tse, Y Wang, CL Zhang, X Cao, CW Lau, J Huang, L He, JY Luo, YT Shih, S Wan, CF Ng, L Wang, RCW Ma, JJ Chiu, TF Chan, X Yu Tian, Y Huang SOX4 is a novel phenotypic regulator of endothelial cells in atherosclerosis revealed by single-cell analysis Journal of advanced research, 2022-03-01;43(0):187-203. 2022-03-01 [PMID: 36585108] (Organ Culture, Mouse) Organ Culture Mouse
S Zia, BP Hammond, M Zirngibl, A Sizov, CS Baaklini, SP Panda, MFS Ho, KV Lee, A Mainali, MK Burr, S Williams, AV Caprariell, C Power, T Simmen, BJ Kerr, JR Plemel Single-cell microglial transcriptomics during demyelination defines a microglial state required for lytic carcass clearance Molecular Neurodegeneration, 2022-12-13;17(1):82. 2022-12-13 [PMID: 36514132] (Bioassay, Mouse) Bioassay Mouse
YK Kim, JA Walters, ND Moss, KL Wells, R Sheridan, JG Miranda, RKP Benninger, LL Pyle, RM O'Brien, L Sussel, HW Davidson Zinc Transporter 8 Haploinsufficiency Protects Against Beta Cell Dysfunction in Type 1 Diabetes by Increasing Mitochondrial Respiration Molecular Metabolism, 2022-11-05;66(0):101632. 2022-11-05 [PMID: 36347424] (Bioassay, Mouse) Bioassay Mouse
Show All 252 Publications.

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By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
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Bioinformatics

Gene Symbol Il1b
Uniprot