Recombinant Mouse EphA4 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphA4, also known as Hek8, Tyro1, and Sek, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 is unusual in its ability to be activated by both Ephrin-A and -B molecules, although its interactions with Ephrin-B2 and -B3 are weaker than with Ephrin-A ligands (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 528 amino acid (aa) extracellular domain (ECD) of mouse EphA4 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 417 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Within the ECD, mouse EphA4 shares 98.3% and 99.6% aa sequence identity with human and rat EphA4, respectively. Alternate splicing of mouse EphA4 generates a short isoform with a 50 aa deletion within the kinase domain. EphA4 is activated by interactions with Ephrin ligands, triggering a repulsive effect on neurite migration (6, 7). This function is important for the accurate guidance and pathfinding of neurites and axons in the spiral ganglion of the cochlea, the anterior commissure, and the corticospinal tract (6‑8). Neuronal EphA4 interactions with astrocyte‑expressed Ephrins also plays a critical role in long term potentiation by regulating hippocampal neuron dendrite arborization, spine maturation, and function (9‑11). The up‑regulation of EphA4 in gastric carcinoma is negatively correlated with patient survival (12). In glioma, EphA4 associates with FGF R1, and this enhances FGF basic-induced tumor cell migration (13). EphA4 is also involved in morphogenesis of the thymic epithelium and T cell development (14).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Adhesion Assay, Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ICC, Western Blot Control.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using 641-A4	Applications	Species
J Defourny, C Audouard, A Davy, M Thiry Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea Brain research bulletin, 2021-06-15;174(0):153-160. 2021-06-15 [PMID: 34139316] (Cell Culture, Mouse)	Cell Culture	Mouse
S Gu, WY Fu, AKY Fu, EPS Tong, FCF Ip, X Huang, NY Ip Identification of new EphA4 inhibitors by virtual screening of FDA-approved drugs Sci Rep, 2018-05-09;8(1):7377. 2018-05-09 [PMID: 29743517] (ELISA (Capture))	ELISA (Capture)
L Schoonaert, L Rué, B Roucourt, M Timmers, S Little, L Chávez-Gut, M Dewilde, P Joyce, A Curnock, P Weber, J Haustraete, G Hassanzade, B De Stroope, L Van Den Bo, P Van Damme, R Lemmens, W Robberecht Identification and characterization of Nanobodies targeting the EphA4 receptor J. Biol. Chem., 2017-05-19;292(27):11452-11465. 2017-05-19 [PMID: 28526745] (Bioassay, Human)	Bioassay	Human
Weiss A, Airik R, Bohnenpoll T, Greulich F, Foik A, Trowe M, Rudat C, Costantini F, Adams R, Kispert A Nephric duct insertion requires EphA4/EphA7 signaling from the pericloacal mesenchyme. Development, 2014-09-01;141(17):3420-30. 2014-09-01 [PMID: 25139858] (Bioassay, Mouse)	Bioassay	Mouse
Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells. J Immunol, 2014-03-31;192(9):4184-91. 2014-03-31 [PMID: 24688023] (Bioassay, Mouse)	Bioassay	Mouse
Han , Xiaofeng, Xu , Yan, Yang , Yilei, Xi , Jingle, Tian , Wang, Duggineni , Srinivas, Huang , Ziwei, An , Jing Discovery and characterization of a novel cyclic peptide that effectively inhibits ephrin binding to the EphA4 receptor and displays anti-angiogenesis activity. PLoS ONE, 2013-11-12;8(11):e80183. 2013-11-12 [PMID: 24265799] (Adhesion Assay, Human)	Adhesion Assay	Human
Stiffel V, Amoui M, Sheng M, Mohan S, Lau K EphA4 receptor is a novel negative regulator of osteoclast activity. J Bone Miner Res, 2014-04-01;29(4):804-19. 2014-04-01 [PMID: 23983218] (Bioassay, Mouse)	Bioassay	Mouse
EphA7 Expression Identifies a Unique Neuronal Compartment in the Rat Striatum [PMID: 23348681] (Western Blot Control, Mouse)	Western Blot Control	Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010-03-24;30(12):4246-55. 2010-03-24 [PMID: 20335460] (Bioassay, Rat)	Bioassay	Rat
Petros TJ, Williams SE, Mason CA Temporal regulation of EphA4 in astroglia during murine retinal and optic nerve development. Mol. Cell. Neurosci., 2006-03-30;32(1):49-66. 2006-03-30 [PMID: 16574431] (ICC, Mouse)	ICC	Mouse
Murai KK, Nguyen LN, Koolpe M, McLennan R, Krull CE, Pasquale EB Targeting the EphA4 receptor in the nervous system with biologically active peptides. Mol. Cell. Neurosci., 2003-12-01;24(4):1000-11. 2003-12-01 [PMID: 14697664] (Binding Assay, Bioassay, Mouse)	Binding Assay, Bioassay	Mouse
Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci., 2003-02-01;6(2):153-60. 2003-02-01 [PMID: 12496762] (Bioassay, Mouse)	Bioassay	Mouse
Show All 12 Publications. Collapse Publications.

Array 641-A4

• EphA4 Antibodies
• EphA4 Proteins and Enzymes