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Recombinant Human IFN-alpha A (alpha 2a) Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-alpha A (alpha 2a) Protein Summary

Details of Functionality

Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Source
E. coli-derived human IFN-alpha A protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
IFNA2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
11100-1 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Buffer

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha A (alpha 2a) Protein

  • alpha-2a interferon
  • Ifa2
  • IFNA
  • IFNA2
  • IFNA2a
  • IFNA2b
  • IFNA2c
  • IFNAA
  • IFNalpha 2
  • IFN-alpha 2
  • IFN-alpha-2
  • IFN-alphaA
  • INFA2
  • interferon alpha 2b
  • interferon alpha A
  • interferon alpha-2
  • Interferon alpha-A
  • interferon, alpha 2
  • LeIF A
  • MGC125764
  • MGC125765

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

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Recombinant Human
IFN-alpha A (alpha 2a ...
11100-1
Recombinant Human IFN-alpha A (alpha 2a) Protein
Species: Hu
Applications: Bioactivity

Publications for IFN-alpha 2 (11100-1)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Cell Culture, Control.


Filter By Application
Bioassay
(12)
Cell Culture
(2)
Control
(1)
All Applications
Filter By Species
Human
(15)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 11100-1 Applications Species
Prelli Bozzo, C;Laliberté, A;De Luna, A;Pastorio, C;Regensburger, K;Krebs, S;Graf, A;Blum, H;Volcic, M;Sparrer, KMJ;Kirchhoff, F; Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef Nature communications 2024-05-07 [PMID: 38714682] (Bioassay, Human) Bioassay Human
TE Hoornweg, GJ Godeke, MN Hoogerwerf, PB van Kaster, A de Vries, H Sprong, GMGM Verjans, D van Riel, JHJ Reimerink, B Rockx, CBEM Reusken Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands Scientific Reports, 2023-02-18;13(1):2872. 2023-02-18 [PMID: 36807371] (Bioassay, Human) Bioassay Human
C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta , L Wettstein, T Weil, J Wei beta , F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro Nature Communications, 2021-07-28;12(1):4584. 2021-07-28 [PMID: 34321474] (Bioassay, Human) Bioassay Human
J Xing, A Zhang, Y Du, M Fang, LJ Minze, YJ Liu, XC Li, Z Zhang Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells Nature Communications, 2021-05-11;12(1):2681. 2021-05-11 [PMID: 33976210] (Bioassay, Human) Bioassay Human
C Blume, CL Jackson, CM Spalluto, J Legebeke, L Nazlamova, F Conforti, JM Perotin, M Frank, J Butler, M Crispin, J Coles, J Thompson, RA Ridley, LSN Dean, M Loxham, S Reikine, A Azim, K Tariq, DA Johnston, PJ Skipp, R Djukanovic, D Baralle, CJ McCormick, DE Davies, JS Lucas, G Wheway, V Mennella A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection Nature Genetics, 2021-01-11;0(0):. 2021-01-11 [PMID: 33432184] (Bioassay, Human) Bioassay Human
G Maarifi, N Smith, S Maillet, O Moncorgé, C Chamontin, J Edouard, F Sohm, FP Blanchet, JP Herbeuval, G Lutfalla, JP Levraud, NJ Arhel, S Nisole TRIM8 is required for virus-induced IFN response in human plasmacytoid dendritic cells Sci Adv, 2019-11-20;5(11):eaax3511. 2019-11-20 [PMID: 31799391] (Cell Culture, Human) Cell Culture Human
RK Powers, R Culp-Hill, MP Ludwig, KP Smith, KA Waugh, R Minter, KD Tuttle, HC Lewis, AL Rachubinsk, RE Granrath, M Carmona-Ir, RB Wilkerson, DE Kahn, M Joshi, A Lleó, R Blesa, J Fortea, A D'Alessand, JC Costello, KD Sullivan, JM Espinosa Trisomy 21 activates the kynurenine pathway via increased dosage of interferon receptors Nat Commun, 2019-10-18;10(1):4766. 2019-10-18 [PMID: 31628327] (Cell Culture, Human) Cell Culture Human
JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study PLoS ONE, 2017-06-30;12(6):e0179981. 2017-06-30 [PMID: 28665954] (Control, Human) Control Human
Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production Proc Natl Acad Sci USA, 2016-09-06;113(38):10625-10630. 2016-09-06 [PMID: 27601648] (Bioassay, Human) Bioassay Human
AM Gomez, M Ouellet, A Deshiere, Y Breton, MJ Tremblay HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status J Immunol, 2016-03-28;0(0):. 2016-03-28 [PMID: 27022194] (Bioassay, Human) Bioassay Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol IFNA2
Uniprot