Recombinant Human IFN-alpha-2B Protein demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 3.00-60.0 pg/mL
2 μg/lane of Recombinant Human IFN-alpha-2B Protein (Catalog # 11013-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more
Recombinant Human IFN-alpha-2B Protein, CF Summary
Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 fot this effect is 3.00-60.0 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived human IFN-alpha protein Cys24-Glu188
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-22 kDa, under reducing conditions.
Publications
Read Publications using 11013-IF in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IFN-alpha-2B Protein, CF
IFNA
IFNA2
IFNA2b
IFNalpha
IFN-alpha
IFN-alphaA
INFA2
interferon alpha 2
LeIF D
Background
Interferons (IFN) are a family of
cytokines with potent antiviral, antiproliferative and immunomodulatory
properties, classified based on their binding specificity to cell surface
receptors (1). There are more than a dozen closely related IFN alpha subtypes
found in both the human and mouse genome, each sharing about 80% amino
acid (aa) sequence homology (2, 3). There are several variants of human
IFNA2, IFNA2a, IFNA2b and IFNA2c, which only differ by a few point mutations
(4). The extracellular domain (ECD) of mature human IFNA2b shares 59% aa
sequence identity with mouse IFNA2. The type I IFNs bind to the interferon
alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit)
and IFNAR2 (beta -subunit) (5, 6). While individual IFN alpha
subtypes are known to display unique efficacies to viral protection,
nothing is known about functions unique to IFNA2 in human physiology. However,
IFNA2 has become the
representative type I IFN subtype used in basic research and numerous clinical
applications (7). Since being first approved for treatment of hairy cell
leukemia in 1986, IFNA2b has been approved for the treatment of several
malignancies including AIDS-related Kaposi's sarcoma, malignant
melanoma and chronic hepatitis B and C (8). More
recently, human IFNA2b has been tested as a potential treatment for COVID-19 (9).
Pestka S. et al. (1987) Annu Rev Biochem. 56:727.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
Lee, N. et al. (1995) J. Interferon Cytokine Res. 15:341.
STING in Innate Immunity and Cancer: What’s the Buzz About? STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ... Read full blog post.
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