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Recombinant Human IFN-alpha-2A Protein, CF

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Recombinant Human IFNA-2A (Catalog # 10984-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 3.00-30.0 pg/mL
Recombinant Human IFNA-2A (Catalog # 10984-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 3.00-30.0 ...read more
2 μg/lane of Recombinant Human IFN-alpha-2A Protein (Catalog # 10984-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
10984-IF-050
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Recombinant Human IFN-alpha-2A Protein, CF Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 fot this effect is 3.00-30.0 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived human IFN-alpha protein
Cys24-Glu188
Accession #
N-terminal Sequence
Cys24
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-24 kDa, under reducing conditions.
Publications
Read Publication using
10984-IF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha-2A Protein, CF

  • IFNA
  • IFNA2
  • IFNA2b
  • IFNalpha
  • IFN-alpha
  • IFN-alphaA
  • INFA2
  • interferon alpha 2
  • LeIF D

Background

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80%amino acid (aa) sequence homology (2 - 4). There are several variants of human IFNA2, IFNA2a, IFNA2b and IFNA2c, which only differ by a few point mutations (5). The extracellular domain (ECD) of mature human IFNA2A, shares 59% aa sequence identity with mouse IFNA2. The type I IFNs bind to t e interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). While individual IFN alpha subtypes are known to display unique efficacies to viral protection, nothing is known about functions unique to IFNA2 in human physiology. However, IFNA2 has become the representative type I IFN subtype used in basic research and numerous clinical applications (8). Since being first approved for treatment of hairy cell leukemia in 1986, IFNA2a has been approved for the treatment of several malignancies including AIDS-related Kaposi's sarcoma, chronic hepatitis C and chronic myelogenous leukemia (9).
  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Lee, N. et al. (1995) J. Interferon Cytokine Res. 15:341.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Paul, F. et al. (2015) Gene. 567:132.
  9. Kiladjian, J.J. et al. (2008) Leukemia 22:1990.

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Recombinant Human
IFN-alpha-2A Protein, ...
10984-IF
Recombinant Human IFN-alpha-2A Protein, CF
Species: Hu
Applications: Bioactivity

Publications for IFN-alpha (10984-IF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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