Recombinant Human 5T4 Fc Chimera Avi-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Mature 5T4 consists of an extracellular domain (ECD) with eight leucine-rich repeats thought to antagonize Wnt signaling, a single transmembrane segment, and a short cytoplasmic tail with a PDZ-binding motif (2). Within the ECD, human 5T4 shares 78% and 77% amino acid sequence identity with mouse and rat 5T4, respectively. 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). It has been identified that 5T4 interacts with TIP-2/GIPC, a common interacting protein involved in cancer, through its PDZ domain (6). 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibits Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1). In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8. Our Avi-tag Biotinylated 5T4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Array AVI4975

• 5T4 Antibodies
• 5T4 Lysate
• 5T4 Protein
• 5T4 Proteins and Enzymes