![Western Blot: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody (M01), clone 3A3.Lane 1: PUS1 transfected lysate(44.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PUS1-Antibody-3A3-Western-Blot-H00080324-M01-img0004.jpg "Western Blot: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody (M01), clone 3A3.Lane 1: PUS1 transfected lysate(44.4 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 3A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | PUS1 (AAH02901, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD |
| Specificity | Reacts with pseudouridylate synthase 1. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PUS1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is reactive against recombinant protein in western blot and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of monoclonal antibody to PUS1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PUS1-Antibody-3A3-Immunocytochemistry-Immunofluorescence-H00080324-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of monoclonal antibody to PUS1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunoprecipitation: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PUS1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PUS1-Antibody-3A3-Immunoprecipitation-H00080324-M01-img0003.jpg "Immunoprecipitation: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PUS1 MaxPab rabbit polyclonal antibody.")
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