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PSMA1 Antibody

Images

 
Independent Antibodies: Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).
Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody [NBP1-81768] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Western Blot: PSMA1 Antibody [NBP1-81768] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.
Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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PSMA1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Proteasome 20S C2 Protein (NBP1-81768PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMA1 Antibody

  • HC2;HEL-S-275;NU;PROS30;Proteasome subunit alpha type;Proteasome subunit alpha type-1
  • Macropain Subunit C2
  • P30-33K
  • PROS30
  • Proteasome Component C2
  • Proteasome nu Chain
  • PSC2
  • PSMA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMA1 Antibody (NBP1-81768) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMA1
Uniprot