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PSGL-1/CD162 Antibody (PSGL1/1601)

Images

 
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) [NBP2-53344] - Formalin-fixed, paraffin-embedded human Tonsil stained with PSGL-1/CD162 Antibody (PSGL1/1601).
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) [NBP2-53344] - Formalin-fixed, paraffin-embedded human Spleen stained withCD162 Monoclonal Antibody (PSGL1/1601).
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (PSGL1/1601) [NBP2-53344] - Formalin-fixed, paraffin-embedded human Tonsil stained withCD162 Monoclonal Antibody (PSGL1/1601).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
PSGL1/1601
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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PSGL-1/CD162 Antibody (PSGL1/1601) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54380)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human PSGL-1/CD162 protein (Uniprot: Q14242)
Localization
Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SELPLG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PSGL-1/CD162 Antibody (PSGL1/1601)

  • CD162 antigen
  • CD162
  • P-selectin glycoprotein ligand 1
  • PSGL1
  • PSGL-1
  • PSGL-1cutaneous lymphocyte-associated associated antigen
  • selectin P ligandCLA
  • SELPLG

Background

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SELPLG
Uniprot