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PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

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Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341] - Formalin-fixed, paraffin-embedded human Testis stained with gp100 Mouse Monoclonal Antibody (PMEL/783).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341] - Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/783).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
PMEL/783
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted Summary

Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Immunogen
Recombinant human PMEL17/SILV protein (Uniprot: P40967)
Localization
Cytoplasmic
Marker
Melanoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

  • D12S53EP1
  • Gp100
  • ME20
  • ME20M
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL
  • PMEL17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • SILV
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PMEL