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PMEL17/SILV Antibody (1067144) [Unconjugated]

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PMEL was detected in immersion fixed paraffin-embedded sections of Human Melanoma using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11439) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
PMEL was detected in immersion fixed paraffin-embedded sections of Human Melanoma using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11439) at 5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
1067144
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB11439-SP
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PMEL17/SILV Antibody (1067144) [Unconjugated] Summary

Immunogen
Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967
Specificity
Detects human PMEL in direct ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PMEL17/SILV Antibody (1067144) [Unconjugated]

  • D12S53EP1
  • Gp100
  • ME20
  • ME20M
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL
  • PMEL17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • SILV
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Premelanasome protein (PMEL) is a nonmutated "self" antigen expressed by melanocytes, most melanoma cells, substantia nigra, and retinal pigmented epithelium.  This protein plays an essential role in the structural organization of premelanosomes. PMEL is over-expressed in all stages of melanoma progression and is negative prognostic marker for the overall survival rate of melanoma patients.  PMEL is also associated with melanin-based pigmentation, neurodegenerative disease (functional amyloid) and glaucoma (missense mutations). How PMEL amyloid is tolerated within cells is of major therapeutic interest as it is related to Alzheimer's and Parkinson's disease.
  1. Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
    doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232.

  2. Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun 9;26(12):3529.
    doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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