We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Name: PKC alpha Antibody (133) [PerCP]
Brand: Novus Biologicals
SKU: NBP3-08229PCP
Price: 619 USD

Question

We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue.  I see that you have many antibodies, including several monoclonals.  I wanted to confirm with you that the following antibodies are specific for C-alpha.  NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143.  Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

Accepted Answer

We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, IHC
Clone	133
Clonality	Monoclonal
Host	Mouse
Conjugate	PerCP

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-08229AF350

Alexa Fluor 405 NBP3-08229AF405

Alexa Fluor 488 NBP3-08229AF488

Alexa Fluor 532 NBP3-08229AF532

Alexa Fluor 594 NBP3-08229AF594

Alexa Fluor 647 NBP3-08229AF647

Alexa Fluor 700 NBP3-08229AF700

Alexa Fluor 750 NBP3-08229AF750

Allophycocyanin NBP3-08229APC

Biotin NBP3-08229B

CoraFluor 1 NBP3-08229CL1

DyLight 350 NBP3-08229UV

DyLight 405 NBP3-08229V

DyLight 488 NBP3-08229G

DyLight 550 NBP3-08229R

DyLight 594 NBP3-08229DL594

DyLight 650 NBP3-08229C

DyLight 680 NBP3-08229FR

DyLight 755 NBP3-08229IR

FITC NBP3-08229F

HRP NBP3-08229H

Janelia Fluor 525 NBP3-08229JF525

Janelia Fluor 549 NBP3-08229JF549

Janelia Fluor 585 NBP3-08229JF585

Janelia Fluor 635 NBP3-08229JF635

Janelia Fluor 646 NBP3-08229JF646

Janelia Fluor 669 NBP3-08229JF669

PE NBP3-08229PE

Unconjugated NBP3-07334-100ug

Catalog# & Formulation	Size	Price
Standard Formulation NBP3-07334-100ug

Azide and BSA Free NBP3-08229

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-08229AF350
Alexa Fluor 405	NBP3-08229AF405
Alexa Fluor 488	NBP3-08229AF488
Alexa Fluor 532	NBP3-08229AF532
Alexa Fluor 594	NBP3-08229AF594
Alexa Fluor 647	NBP3-08229AF647
Alexa Fluor 700	NBP3-08229AF700
Alexa Fluor 750	NBP3-08229AF750
Allophycocyanin	NBP3-08229APC
Biotin	NBP3-08229B
CoraFluor 1	NBP3-08229CL1
DyLight 350	NBP3-08229UV
DyLight 405	NBP3-08229V
DyLight 488	NBP3-08229G
DyLight 550	NBP3-08229R
DyLight 594	NBP3-08229DL594
DyLight 650	NBP3-08229C
DyLight 680	NBP3-08229FR
DyLight 755	NBP3-08229IR
FITC	NBP3-08229F
HRP	NBP3-08229H
Janelia Fluor 525	NBP3-08229JF525
Janelia Fluor 549	NBP3-08229JF549
Janelia Fluor 585	NBP3-08229JF585
Janelia Fluor 635	NBP3-08229JF635
Janelia Fluor 646	NBP3-08229JF646
Janelia Fluor 669	NBP3-08229JF669
PE	NBP3-08229PE
Unconjugated	NBP3-07334-100ug

PKC alpha Antibody (133) [PerCP]

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Immunogen	Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCalpha. (Uniprot: P17252)
Localization	Cytoplasm, Cell Membrane, Nucleus
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	PRKCA
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	PBS
Preservative	0.05% Sodium Azide
Purity	Protein A or G purified