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PKC alpha Antibody (133) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
133
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08229AF350
Alexa Fluor 405
NBP3-08229AF405
Alexa Fluor 488
NBP3-08229AF488
Alexa Fluor 532
NBP3-08229AF532
Alexa Fluor 594
NBP3-08229AF594
Alexa Fluor 647
NBP3-08229AF647
Alexa Fluor 700
NBP3-08229AF700
Alexa Fluor 750
NBP3-08229AF750
Allophycocyanin
NBP3-08229APC
Biotin
NBP3-08229B
CoraFluor 1
NBP3-08229CL1
DyLight 350
NBP3-08229UV
DyLight 405
NBP3-08229V
DyLight 488
NBP3-08229G
DyLight 550
NBP3-08229R
DyLight 594
NBP3-08229DL594
DyLight 650
NBP3-08229C
DyLight 680
NBP3-08229FR
DyLight 755
NBP3-08229IR
FITC
NBP3-08229F
HRP
NBP3-08229H
Janelia Fluor 549
NBP3-08229JF549
Janelia Fluor 585
NBP3-08229JF585
Janelia Fluor 635
NBP3-08229JF635
Janelia Fluor 646
NBP3-08229JF646
Janelia Fluor 669
NBP3-08229JF669
PE
NBP3-08229PE
PerCP
NBP3-08229PCP
Unconjugated
NBP3-07334-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07334-100ug
Azide and BSA Free
NBP3-08229
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PKC alpha Antibody (133) [Janelia Fluor® 525] Summary

Immunogen
Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCalpha. (Uniprot: P17252)
Localization
Cytoplasm, Cell Membrane, Nucleus
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRKCA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PKC alpha Antibody (133) [Janelia Fluor® 525]

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Background

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). All PKC isotypes share a characteristic equence motif C1 in addition to a serine/threonine-protein kinase domain. The cPKC isotypes include PKC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PKC alpha Antibody (NBP3-08229JF525). (Showing 1 - 1 of 1 FAQ).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCA