![Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.](http://images.novusbio.com/fullsize/PDE10A-Antibody-Western-Blot-NB300-645-img0005.jpg "Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.7 mg/ml |
| Immunogen | Synthetic peptide taken within amino acid region 300-350 on human PDE10A2 protein common to all PDE10A variants. |
| Localization | Located mostly to soluble cellular fractions. |
| Specificity | This has no cross-reactivity against any other PDE family members tested so far. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDE10A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF reactivity reported in (PMID: 21559457). Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%. |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.7 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain.](http://images.novusbio.com/fullsize/PDE10A-Antibody-Western-Blot-NB300-645-img0001.jpg "Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain.")
![Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645] - Slide A is negative control. Slide B is stained with NB300-645 at 1:200 antibody dilution in DiluObuffer.](http://images.novusbio.com/fullsize/PDE10A-Antibody-Immunohistochemistry-Paraffin-NB300-645-img0003.jpg "Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645] - Slide A is negative control. Slide B is stained with NB300-645 at 1:200 antibody dilution in DiluObuffer.")
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![N/A CREB [p Ser133] [Biotin]](https://images.novusbio.com/images2/dyc2510-2_human-mouse-rat-phospho-creb-s133-duoset-ic-elisa-2-plate-712021221551.png)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")