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p63/TP73L Antibody (CL3748)

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Orthogonal Strategies: Western Blot: p63/TP73L Antibody (CL3748) [NBP3-05501] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human prostate shows nuclear immunoreactivity in basal epithelial cells.
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human liver shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human lung cancer (adenocarcinoma) shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human placenta shows nuclear positivity in a subset of cells.
Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human prostate cancer shows absence of nuclear immunoreactivity in tumor cells, while it is present in the adjacent normal a basal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL3748
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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p63/TP73L Antibody (CL3748) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: CACPGRDRKADEDSIRKQQVSDSTKNGDAFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQ
Epitope
MQYLPQHTIETYRQQ
Predicted Species
Mouse (99%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TP63
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for p63/TP73L Antibody (CL3748)

  • amplified in squamous cell carcinoma
  • Chronic ulcerative stomatitis protein
  • CUSP
  • EEC3
  • EEC3B(p51B)
  • Keratinocyte transcription factor KET
  • KET
  • KETRHS
  • LMS
  • OFC8
  • OFC8SHFM4TP73LNBP
  • p40
  • p51
  • p51tumor protein p53-like
  • p53CP
  • p63
  • p63AIS
  • p73H
  • p73L
  • p73LB(p51A)
  • SHFM4
  • TP53CP
  • TP53L
  • TP63
  • TP73L
  • Transformation-related protein 63
  • tumor protein 63
  • tumor protein p53-competing protein
  • tumor protein p63 deltaN isoform delta
  • tumor protein p63
  • Tumor protein p73-like

Background

The p63 gene, a homologue of the tumor suppressor p53, is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 and to the related p73 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TP63