![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0004.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human p130Cas/BCAR1 (phospho Tyr165). The exact sequence is proprietary. |
| Modification | p Tyr165 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BCAR1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for p130Cas/BCAR1 Antibody (NBP3-13075)Find related products by research area.
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| Gene Symbol | BCAR1 |
![Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - p130Cas/BCAR1 (phospho Tyr165) antibody detects p130Cas/BCAR1 (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas/BCAR1 (phospho Tyr165) protein stained by p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-[p-Tyr165]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13075-img0002.jpg "Immunocytochemistry/Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - p130Cas/BCAR1 (phospho Tyr165) antibody detects p130Cas/BCAR1 (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas/BCAR1 (phospho Tyr165) protein stained by p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)](http://images.novusbio.com/fullsize/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0001.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)")
![Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p130Cas-BCAR1-[p-Tyr165]-Antibody-Western-Blot-NBP3-13075-img0003.jpg "Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")