![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0012.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clone | 1C12-2A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | NFIC (AAH12120, 1 a.a. ~ 428 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPSWYLG |
| Specificity | NFIC - nuclear factor I/C (CCAAT-binding transcription factor) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFIC |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
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| Publication using H00004782-M01 | Applications | Species |
|---|---|---|
| Lamani E, Gluhak-Heinrich J, MacDougall M. NFI-C2 temporal-spatial expression and cellular localization pattern during tooth formation. Dev Growth Differ 2015-12-21 [PMID: 26687982] |
Secondary Antibodies |
Isotype Controls |
Research Areas for NFIC Antibody (H00004782-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunocytochemistry-Immunofluorescence-H00004782-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Immunohistochemistry-Paraffin-H00004782-M01-img0009.jpg "Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.")
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0010.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi ( Cat # H00004782-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody (M01), clone 1C12-2A2 (Cat # H00004782-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - NFIC monoclonal antibody (M01), clone 1C12-2A2 Analysis of NFIC expression in A-431.](http://images.novusbio.com/fullsize/NFIC-Antibody-1C12-2A2-Western-Blot-H00004782-M01-img0011.jpg "Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - NFIC monoclonal antibody (M01), clone 1C12-2A2 Analysis of NFIC expression in A-431.")
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