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NCAM-1/CD56 Antibody (123C3.D5) [PE/Cy7]

 
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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC
Clone
123C3.D5
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33132AF350
Alexa Fluor 405
NBP2-33132AF405
Alexa Fluor 488
NBP2-33132AF488
Alexa Fluor 532
NBP2-33132AF532
Alexa Fluor 594
NBP2-33132AF594
Alexa Fluor 647
NBP2-33132AF647
Alexa Fluor 700
NBP2-33132AF700
Alexa Fluor 750
NBP2-33132AF750
Allophycocyanin
NBP2-33132APC
Allophycocyanin/Cy7
NBP2-33132APCCY7
Biotin
NBP2-33132B
DyLight 350
NBP2-33132UV
DyLight 405
NBP2-33132V
DyLight 488
NBP2-33132G
DyLight 550
NBP2-33132R
DyLight 594
NBP2-33132DL594
DyLight 650
NBP2-33132C
DyLight 680
NBP2-33132FR
DyLight 755
NBP2-33132IR
FITC
NBP2-33132F
HRP
NBP2-33132H
Janelia Fluor 549
NBP2-33132JF549
Janelia Fluor 646
NBP2-33132JF646
PE
NBP2-33132PE
PE/Atto594
NBP2-33132PEATT594
PE/Cy5.5
NBP2-33132PECY55
PerCP
NBP2-33132PCP
Unconjugated
NBP2-15184-0.1mg
mFluor Violet 450 SE
NBP2-33132MFV450
mFluor Violet 500 SE
NBP2-33132MFV500
mFluor Violet 610 SE
NBP2-33132MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-15184-0.1mg
Azide and BSA Free
NBP2-33132
IHC-Prediluted
NBP2-44711
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NCAM-1/CD56 Antibody (123C3.D5) [PE/Cy7] Summary

Immunogen
Membrane preparation of a small cell lung carcinoma
Localization
Cell surface
Marker
Neuronal Cell Marker
Specificity
This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCAM1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for NCAM-1/CD56 Antibody (123C3.D5) [PE/Cy7]

  • CD56 / NCAM-1
  • CD56 antigen
  • CD56
  • MSK39
  • NCAM1
  • N-CAM-1
  • NCAM-1
  • NCAMantigen recognized by monoclonal 5.1H11
  • neural cell adhesion molecule 1
  • neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NCAM-1/CD56
Antibody (123C3.D5) [ ...
NBP2-33132PECY7
NCAM-1/CD56 Antibody (123C3.D5) [PE/Cy7]
Species: Hu, Rt
Applications: Simple Western, Flow, ICC/IF, IHC

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NCAM1