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Human Myoglobin ELISA Kit (Chemiluminescence)

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ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] - Samples were spiked with high concentrations of Human Myoglobin and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human Myoglobin ELISA Kit (Chemiluminescence) [NBP2-68064] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.63-40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

Human Myoglobin ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed.
Standard Curve Range
0.63-40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 11.08%
Intra-Assay
CV% < 10.26%
Spike Recovery
88-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
MB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Myoglobin ELISA Kit (Chemiluminescence)

  • MB
  • MGC13548
  • Myoglobin
  • PVALB

Background

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MB