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Myoglobin Antibody (4F8)

Images

 
Western Blot: Myoglobin Antibody (4F8) [H00004151-M13] - Analysis of MB expression in transfected 293T cell line by MB monoclonal antibody (M13), clone 4F8. Lane 1: MB transfected lysatE (17.2 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Myoglobin Antibody (4F8) [H00004151-M13] - Analysis of monoclonal antibody to MB on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Myoglobin Antibody (4F8) [H00004151-M13] - Detection limit for recombinant GST tagged MB is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Myoglobin Antibody (4F8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MB (NP_005359.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKI
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
Application Notes
It has been used for ELISA and WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Myoglobin Antibody (4F8)

  • MB
  • MGC13548
  • Myoglobin
  • PVALB

Background

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MB
Uniprot