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Myogenin Antibody (671038) [Unconjugated]

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Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
C2C12 cells were stained with Mouse Anti-Human/Mouse Myogenin Monoclonal Antibody (Catalog # MAB6686, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF
Clone
671038
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Myogenin Antibody (671038) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Specificity
Detects human Myogenin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB6686 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myogenin Antibody (671038) [Unconjugated]

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Background

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myogenin Antibody (MAB6686)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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(1)
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Bioinformatics

Gene Symbol MYOG
Uniprot