Novus Biologicals products are now on bio-techne.com

MyoD Antibody (SPM427) [mFluor Violet 500 SE]

Images

 
MyoD Antibody (SPM427) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM427
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 500 SE

Order Details

MyoD Antibody (SPM427) [mFluor Violet 500 SE] Summary

Immunogen
Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189. (Uniprot: P15172)
Epitope
aa 180-189
Localization
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Marker
Rhabdomyosarcoma Marker
Specificity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this monoclonal antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MyoD Antibody (SPM427) [mFluor Violet 500 SE]

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB66861
Species: Hu
Applications: ICC, WB
NBP2-44245
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-52030
Species: Hu
Applications: PEP-ELISA, WB
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB1675
Species: Ch, Hu, Mu, Rt
Applications: ICC, IHC
291-G1
Species: Hu
Applications: BA
788-G8/CF
Species: Hu, Mu, Rt
Applications: BA
H00004205-M15
Species: Hu
Applications: ELISA, ICC/IF, WB
MAB2457
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, WB
NBP2-46085
Species: Hu
Applications: IHC, WB
H00027065-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF6116
Species: Hu
Applications: ICC, WB
MAB7650
Species: Mu
Applications: IHC, WB
NBP2-13957
Species: Hu
Applications: IHC, IHC-P, WB
AF4377
Species: Hu, Mu
Applications: ICC, Simple Western, WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP2-34772MFV500
Species: Hu, Mu, Rt, Ch
Applications: Flow, ICC/IF, IHC, CyTOF-ready

Publications for MyoD Antibody (NBP2-34772MFV500) (0)

There are no publications for MyoD Antibody (NBP2-34772MFV500).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MyoD Antibody (NBP2-34772MFV500) (0)

There are no reviews for MyoD Antibody (NBP2-34772MFV500). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for MyoD Antibody (NBP2-34772MFV500) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MyoD Products

Array NBP2-34772MFV500

Research Areas for MyoD Antibody (NBP2-34772MFV500)

Find related products by research area.

Blogs on MyoD

There are no specific blogs for MyoD, but you can read our latest blog posts.
Earn Double Rewards for Data!

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MyoD Antibody (SPM427) [mFluor Violet 500 SE] and receive a gift card or discount.

Bioinformatics

Gene Symbol MYOD1