Novus Biologicals products are now on bio-techne.com

MyoD Antibody (rMYD712) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: MyoD Antibody (rMYD712) [NBP3-08505] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).
Protein Array: MyoD Antibody (rMYD712) [NBP3-08505] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
rMYD712
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

MyoD Antibody (rMYD712) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-07394).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human MyoD protein (Uniprot: P15172)
Localization
Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation
Marker
Rhabdomyosarcoma Marker
Specificity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This monoclonal antibody does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin : 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for MyoD Antibody (rMYD712) - Azide and BSA Free

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56510
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-44245
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-52030
Species: Hu
Applications: PEP-ELISA, WB
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB1675
Species: Ch, Hu, Mu, Rt
Applications: ICC, IHC
291-G1
Species: Hu
Applications: BA
788-G8/CF
Species: Hu, Mu, Rt
Applications: BA
H00004205-M15
Species: Hu
Applications: ELISA, ICC/IF, WB
MAB2457
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow, WB
NBP2-46085
Species: Hu
Applications: IHC, WB
H00027065-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
AF6116
Species: Hu
Applications: ICC, WB
MAB7650
Species: Mu
Applications: IHC, WB
NBP2-13957
Species: Hu
Applications: IHC, IHC-P, WB
AF4377
Species: Hu, Mu
Applications: ICC, Simple Western, WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP3-08505
Species: Hu
Applications: IHC, MA

Publications for MyoD Antibody (NBP3-08505) (0)

There are no publications for MyoD Antibody (NBP3-08505).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MyoD Antibody (NBP3-08505) (0)

There are no reviews for MyoD Antibody (NBP3-08505). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MyoD Antibody (NBP3-08505) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MyoD Products

Diseases for MyoD Antibody (NBP3-08505)

Discover more about diseases related to MyoD Antibody (NBP3-08505).
 

Pathways for MyoD Antibody (NBP3-08505)

View related products by pathway.

PTMs for MyoD Antibody (NBP3-08505)

Learn more about PTMs related to MyoD Antibody (NBP3-08505).
 

Research Areas for MyoD Antibody (NBP3-08505)

Find related products by research area.

Blogs on MyoD

There are no specific blogs for MyoD, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MyoD Antibody (rMYD712) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol MYOD1