![Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Immunohistochemistry-Paraffin-NB100-64803-img0002.jpg "Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 2C7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Human myeloperoxidase |
| Specificity | NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MPO |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Membrane permeabilisation is required for Flow Cytometry. 2C7 recognises myeloperoxidase under nonreducing conditions. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0005.jpg "Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.")
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0003.jpg "Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.")
![Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-2C7-Flow-Cytometry-NB100-64803-img0004.jpg "Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.")
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