MuRF1/TRIM63 Antibody Summary
Description |
Stable for at least 1 year at -20°C. |
Immunogen |
MuRF1/TRIM63 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1/TRIM63. This peptide sequence is highly conserved in rat and mouse MuRF1/TRIM63, and has 50% homology to MuRF2 (TRIM-55). [UniProt# Q969Q1] |
Specificity |
The antibody detects 38 and 30 kDa proteins corresponding to the apparent molecular mass of MuRF1/TRIM63 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TRIM63 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:300
- Western Blot 1:1000
|
Theoretical MW |
30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. |
Buffer |
PBS, 1 mg/ml BSA and 50% glycerol |
Preservative |
0.05% Sodium Azide |
Purity |
Antigen Affinity-purified |
Alternate Names for MuRF1/TRIM63 Antibody
Background
MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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