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MuRF1/TRIM63 Antibody

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Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody [NBP3-23425] - Immunocytochemical labeling of MuRF1/TRIM63 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1/TRIM63 antibody, then ...read more
Western Blot: MuRF1/TRIM63 Antibody [NBP3-23425] - Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1/TRIM63 (C-terminal region) (lanes 1 & 2) or ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MuRF1/TRIM63 Antibody Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
MuRF1/TRIM63 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1/TRIM63. This peptide sequence is highly conserved in rat and mouse MuRF1/TRIM63, and has 50% homology to MuRF2 (TRIM-55). [UniProt# Q969Q1]
Specificity
The antibody detects 38 and 30 kDa proteins corresponding to the apparent molecular mass of MuRF1/TRIM63 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM63
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:300
  • Western Blot 1:1000
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Antigen Affinity-purified

Alternate Names for MuRF1/TRIM63 Antibody

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM63