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MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

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Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded rectum cancer tissues using MuRF1 mouse mAb with DAB staining.
Flow Cytometry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded muscle tissues using MuRF1 mouse mAb with DAB staining.
ELISA: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
7B8E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52516-0.025mg
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MuRF1/TRIM63 Antibody (7B8E1) - BSA Free Summary

Immunogen
Synthesized peptide of human MuRF1/TRIM63 (AA: 293-304).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIM63
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This antibody is Cytof ready.
Theoretical MW
40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol TRIM63