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MCAM/CD146 Antibody (P1H12) - BSA Free

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Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.
Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - A surface stain was performed on SK-MEL-28 cells with MCAM/CD146 Antibody (P1H12) NBP1-43346PCP (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 ...read more
Flow Cytometry: MCAM/CD146 Antibody (P1H12) [NBP1-43346] - Analysis using the FITC conjugate of NBP1-43346. Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (blue ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Rb, Rt(-)Species Glossary
Applications WB, Flow, IHC, IP
Clone
P1H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43346AF350
Alexa Fluor 405
NBP1-43346AF405
Alexa Fluor 488
NBP1-43346AF488
Alexa Fluor 532
NBP1-43346AF532
Alexa Fluor 594
NBP1-43346AF594
Alexa Fluor 647
NBP1-43346AF647
Alexa Fluor 700
NBP1-43346AF700
Alexa Fluor 750
NBP1-43346AF750
Allophycocyanin
NBP1-43346APC
Allophycocyanin/Cy7
NBP1-43346APCCY7
Biotin
NBP1-43346B
CoraFluor 1
NBP1-43346CL1
DyLight 350
NBP1-43346UV
DyLight 405
NBP1-43346V
DyLight 488
NBP1-43346G
DyLight 550
NBP1-43346R
DyLight 594
NBP1-43346DL594
DyLight 650
NBP1-43346C
DyLight 680
NBP1-43346FR
DyLight 755
NBP1-43346IR
FITC
NBP1-43346F
HRP
NBP1-43346H
Janelia Fluor 525
NBP1-43346JF525
Janelia Fluor 549
NBP1-43346JF549
Janelia Fluor 585
NBP1-43346JF585
Janelia Fluor 635
NBP1-43346JF635
Janelia Fluor 646
NBP1-43346JF646
Janelia Fluor 669
NBP1-43346JF669
PE
NBP1-43346PE
PE/Atto594
NBP1-43346PEATT594
PE/Cy5.5
NBP1-43346PECY55
PE/Cy7
NBP1-43346PECY7
PerCP
NBP1-43346PCP
mFluor Violet 450 SE
NBP1-43346MFV450
mFluor Violet 500 SE
NBP1-43346MFV500
mFluor Violet 610 SE
NBP1-43346MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-43346-0.025mg
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MCAM/CD146 Antibody (P1H12) - BSA Free Summary

Additional Information
Clone P1H12 was used by HLDA to establish CD designation.
Immunogen
The immunogen for this antibody was CD146.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Publications
Read Publications using
NBP1-43346 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for MCAM/CD146 Antibody (P1H12) - BSA Free

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

The monoclonal antibody P1H12 recognizes human CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (NBP1-43346)(3)

We have publications tested in 2 confirmed species: Rat, Canine.

We have publications tested in 3 applications: FLOW, IF/IHC, IHC-P.


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(1)
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FAQs for MCAM/CD146 Antibody (NBP1-43346). (Showing 1 - 1 of 1 FAQs).

  1. Hello, I am looking at your antibody NBP1-43346APC, and I am curious what part of CD146 this antibody reacts to?
    • The immunogen for clone P1H12 was Human Umbilical Vein Cells. Unfortunately the epitope was not mapped. We don't have any special instructions for permeabilization so we don't believe it is necessary. 

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MCAM