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MARCO Antibody

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Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF7586-SP
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MARCO Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MARCO
Met79-Val520
Accession # Q9UEW3
Specificity
Detects human MARCO in direct ELISAs and Western blots. In direct ELISA, approximately 10% cross-reactivity with recombinant mouse MARCO is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MARCO
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF7586 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MARCO Antibody

  • macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
  • MARCO
  • member 2
  • SCARA2

Background

MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70 kDa pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520). The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MARCO Antibody
AF7586
MARCO Antibody
Species: Hu
Applications: WB

Publications for MARCO Antibody (AF7586)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol MARCO
Uniprot