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Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF749
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Catalog# & Formulation Size Price
Carrier Free
AF749-SP
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Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Lymphotoxin‑ alpha /TNF‑ beta
Specificity
Detects mouse Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human Lymphotoxin‑ alpha /TNF‑ beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LTA
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF749 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated]

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha , TNF-beta , and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta receptor (LT beta R), which does not bind TNF-beta or TNF-alpha . Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta  R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence.

  1. Aggarwal, B.B. (2003) Nature Rev. Immunol. 3:745.
  2. Browning , J.L. et al. (1993) Cell 72:846.
  3. Browning, J.L. et al. (1995) J. Immunol. 154:33.
  4. Pokholok, D.K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:674.
  5. Aggarwal, B.B. et al. (1985) Nature 318:665.
  6. Crowe, P.D. et al. (1994) Science 264:707.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Tumanov, A.V. et al. (2003) Cytokine & Growth Factor Rev. 14:275.
  9. Roach, D.R. et al. (2001) J. Exp. Med. 193:239.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lymphotoxin-alpha/TNF-beta Antibody (AF749)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol LTA