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IRS1 Antibody

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Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IRS1 at approximately 170 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was ...read more
IRS1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human/Mouse/Rat IRS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3978) at 15 µg/mL overnight ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3978-SP
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IRS1 Antibody Summary

Immunogen
E. coli-derived recombinant human IRS1
Ala801-Ala1020
Accession # P35568
Specificity
Detects human, mouse, and rat IRS1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 Antibody

  • HIRS-1
  • insulin receptor substrate 1
  • IRS1
  • IRS-1

Background

IRS1 (Insulin Receptor Substrate 1) is a cytoplasmic protein that participates in the insulin signaling pathway. It is phosphorylated by the insulin receptor upon binding of insulin and subsequently associates with several SH2-domain-containing proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on IRS1.

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Bioinformatics

Gene Symbol IRS1
Uniprot