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Recombinant Rat IL-6 Protein

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SDS-Page: Rat IL-6 Protein [NBP2-35254]
SDS-Page: Rat IL-6 Protein [NBP2-35254]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35254-10ug
Carrier-Free
NBP2-35254-1mg
Carrier-Free
NBP2-35254-500ug
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Recombinant Rat IL-6 Protein Summary

Description
A single, non-glycosylated polypeptide chain containing 187 amino acids corresponding to IL-6 Source: E. coli

Uniprot ID: P20607

Amino Acid Sequence: FPTSQVRRGD FTEDTTHNRP VYTTSQVGGL ITYVLREILE MRKELCNGNS DCMNSDDALS ENNLKLPEIQ RNDGCFQTGY NQEICLLKIC SGLLEFRFYL EFVKNNLQDN KKDKARVIQS NTETLVHIFK QEIKDSYKIV LPTPTSNALL MEKLESQKEW LRTKTIQLIL KALEEFLKVT MRSTRQT

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
IL6 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL6
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of IL-6 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in 10mM HCl to a concentration of 0.1mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Rat IL-6 Protein

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. IL-16 plays an essential role in the final differentiation of Bcells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The rat IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Ralpha chain (CD126), and the signal-transducing component gp130 (also called CD130). The rat IL-6 shares 40 % and 85 % aa sequence identity with human and mouse IL-6 and it is equally active on human and mouse cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
IL-6 Protein
NBP2-35254
Recombinant Rat IL-6 Protein
Species: Rt
Applications: PAGE, Bioactivity

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FAQs for IL-6 Recombinant Protein (NBP2-35254). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Bioinformatics

Gene Symbol IL6
Uniprot