IL-6 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 7 applications: ELISA Development (Capture), ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 10 of 48. Show All 48 Publications. Collapse Publications.

Publications using AF-406-NA	Applications	Species
Zhou, X;Hao, H;Chen, Y;Cao, W;Zhu, Z;Ni, Y;Liu, Z;Jia, F;Wang, Y;Ji, J;Peng Zhang, ; Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in mice Bioactive materials 2024-04-01 [PMID: 38292409] (IHC, Mouse)	IHC	Mouse
Wenli Liu, Mustafa Yalcinkaya, Inés Fernández Maestre, Malgorzata Olszewska, Patrick B. Ampomah, J. Brett Heimlich et al. Blockade of IL-6 signaling alleviates atherosclerosis in Tet2-deficient clonal hematopoiesis Nature Cardiovascular Research 8/4/2023 [PMID: 37539077]
Shang, M;Ni, L;Shan, X;Cui, Y;Hu, P;Ji, Z;Shen, L;Zhang, Y;Zhou, J;Bing Chen, ;Wang, T;Yu, Q; MTHFD2 reprograms macrophage polarization by inhibiting PTEN Cell reports 2023-05-05 [PMID: 37149861] (IHC, Mouse)	IHC	Mouse
AN Jiang, B Wang, S Wang, K Zhao, H Wu, K Yan, W Wu, W Yang The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model BMC Cancer, 2022-06-17;22(1):663. 2022-06-17 [PMID: 35710408] (IHC, Mouse)	IHC	Mouse
Jennifer M. Petrosino, Jacob Z. Longenecker, Colin D. Angell, Scott A. Hinger, Colton R. Martens, Federica Accornero CCN2 participates in overload-induced skeletal muscle hypertrophy Matrix Biology 2/1/2022 [PMID: 35045313]
Gerlach BD, Ampomah PB, Yurdagul A Et al. Efferocytosis induces macrophage proliferation to help resolve tissue injury Cell metabolism 11/10/2021 [PMID: 34784501]
Beatrice Oelze, Kirsten Elger, Patrik Schadzek, Laura Burmeister, Anika Hamm, Sandra Laggies et al. The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6 Molecular and Cellular Biochemistry 10/1/2021 [PMID: 34052945]
David B Vaught, Alyssa R Merkel, Conor C Lynch, James Edwards, Mohammed Noor Tantawy, Timothy Hilliard et al. EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease JBMR Plus 4/1/2021 [PMID: 33869989]
K Tamura, N Goto-Inoue, K Miyata, Y Furuichi, NL Fujii, Y Manabe Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes PLoS ONE, 2020-08-05;15(8):e0237095. 2020-08-05 [PMID: 32756599] (Western Blot, Mouse)	Western Blot	Mouse
KC Clark, DR Hewett, V Panagopoul, N Plakhova, KS Opperman, AL Bradey, KM Mrozik, K Vandyke, S Mukherjee, GCG Davies, DL Worthley, ACW Zannettino Targeted Disruption of Bone Marrow Stromal Cell-Derived Gremlin1 Limits Multiple Myeloma Disease Progression In Vivo Cancers (Basel), 2020-08-03;12(8):. 2020-08-03 [PMID: 32756430] (Neutralization, Human, Mouse)	Neutralization	Human, Mouse
E Zuccolo, I Badi, F Scavello, I Gambuzza, L Mancinelli, F Macrì, CC Tedesco, F Veglia, AR Bonfigli, F Olivieri, A Raucci The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells Calcification Int J Mol Sci, 2020-06-23;21(12):. 2020-06-23 [PMID: 32585876] (IHC, Mouse)	IHC	Mouse
Chetan P. Hans, Neekun Sharma, Rishabh Dev, Jones M. Blain, Jeff Tonniges, Gunjan Agarwal DAPT, a potent Notch inhibitor regresses actively growing abdominal aortic aneurysm via divergent pathways Clinical Science 6/26/2020 [PMID: 32490531]
J Wang, H Liu, G Xie, W Cai, J Xu Identification of hub genes and key pathways of dietary advanced glycation end products?induced non?alcoholic fatty liver disease by bioinformatics analysis and animal experiments Mol Med Rep, 2019-12-09;21(2):685-694. 2019-12-09 [PMID: 31974594] (IHC, Mouse)	IHC	Mouse
Cuihong Jia, Matthew P. Keasey, Hannah M. Malone, Chiharu Lovins, Theo Hagg Vitronectin mitigates stroke-increased neurogenesis only in female mice and through FAK-regulated IL-6 Experimental Neurology 1/1/2020 [PMID: 31678139]
T Tominaga, S Ma, K Saitou, K Suzuki Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle Nutrients, 2019-06-30;11(7):. 2019-06-30 [PMID: 31262006] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Krisztián Pajer, Tamás Bellák, Heinz Redl, Antal Nógrádi Neuroectodermal Stem Cells Grafted into the Injured Spinal Cord Induce Both Axonal Regeneration and Morphological Restoration via Multiple Mechanisms Journal of Neurotrauma 11/1/2019 [PMID: 31111776]
OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation Oxid Med Cell Longev, 2019-04-10;2019(0):6018180. 2019-04-10 [PMID: 31093317] (Western Blot, Mouse)	Western Blot	Mouse
J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis J Transl Med, 2019-03-15;17(1):84. 2019-03-15 [PMID: 30876479] (IHC-P, Mouse)	IHC-P	Mouse
WJ Su, T Zhang, CL Jiang, W Wang Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus Front Cell Neurosci, 2018-11-13;12(0):412. 2018-11-13 [PMID: 30483062] (Western Blot, Mouse)	Western Blot	Mouse
Y Furuichi, Y Manabe, M Takagi, M Aoki, NL Fujii Evidence for acute contraction-induced myokine secretion by C2C12 myotubes PLoS ONE, 2018-10-24;13(10):e0206146. 2018-10-24 [PMID: 30356272] (Western Blot, Mouse)	Western Blot	Mouse
SH Park, J Rhee, SK Kim, JA Kang, JS Kwak, YO Son, WS Choi, SG Park, JS Chun BATF regulates collagen-induced arthritis by regulating T helper cell differentiation Arthritis Res. Ther., 2018-08-02;20(1):161. 2018-08-02 [PMID: 30071881] (IHC-P, Mouse)	IHC-P	Mouse
LL Xu, T Liu, L Wang, L Li, YF Wu, CC Li, B Di, QD You, ZY Jiang 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation Oxid Med Cell Longev, 2018-05-20;2018(0):3271617. 2018-05-20 [PMID: 29887940] (IHC, Mouse)	IHC	Mouse
C Dempsey Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice Brain Behav. Immun, 2016-12-18;0(0):. 2016-12-18 [PMID: 28003153] (ELISA Development (Capture), Mouse)	ELISA Development (Capture)	Mouse
CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells J Leukoc Biol, 2016-08-11;0(0):. 2016-08-11 [PMID: 27515950] (Neutralization, Mouse)	Neutralization	Mouse
Sakamuri SS, Valente AJ, Siddesha JM et al. TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis. Mol. Cell. Endocrinol. 4/1/2016 [PMID: 27040306]
Rachel Crompton, Helen Williams, David Ansell, Laura Campbell, Kirsty Holden, Sheena Cruickshank et al. Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair Laboratory Investigation 4/1/2016 [PMID: 26855364]
C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism Sci Rep, 2016-01-14;6(0):19404. 2016-01-14 [PMID: 26762853] (Neutralization, Mouse)	Neutralization	Mouse
Braun GS, Nagayama Y, Maruta Y et al. IL-6 Trans-Signaling Drives Murine Crescentic GN. J. Am. Soc. Nephrol. 1/1/2016 [PMID: 26041841]
Guo H, Jin D, Chen X Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation. Mol Endocrinol, 2014-08-15;28(10):1616-28. 2014-08-15 [PMID: 25127375] (Western Blot, Mouse)	Western Blot	Mouse
Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al. Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die Experimental Neurology 11/1/2014 [PMID: 24907401]
Bryce N. Alves, Rachel Tsui, Jonathan Almaden, Maxim N. Shokhirev, Jeremy Davis-Turak, Jessica Fujimoto et al. I kappa B epsilon Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner The Journal of Immunology 4/1/2014 [PMID: 24591377]
Rudraraju R, Jones B, Surman S, Sealy R, Thomas P, Hurwitz J Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A. PLoS ONE, 2014-01-22;9(1):e86554. 2014-01-22 [PMID: 24466150] (Neutralization, Mouse)	Neutralization	Mouse
Uto-Konomi A, Miyauchi K, Ozaki N Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS ONE, 2012-07-05;7(7):e40343. 2012-07-05 [PMID: 22792286] (IHC-Fr, Mouse)	IHC-Fr	Mouse
E.G. Vichaya, E.E. Young, M.A. Frazier, J.L. Cook, C.J. Welsh, M.W. Meagher Social disruption induced priming of CNS inflammatory response to Theiler's virus is dependent upon stress induced IL-6 release Journal of Neuroimmunology 10/28/2011 [PMID: 22000153]
Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells. J. Clin. Invest., 2011-09-19;121(10):3846-59. 2011-09-19 [PMID: 21926464] (Neutralization, Mouse)	Neutralization	Mouse
Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages. J. Clin. Invest., 2011-04-25;121(5):1692-708. 2011-04-25 [PMID: 21519141] (Neutralization, Mouse)	Neutralization	Mouse
Weng TI, Wu HY, Lin PY, Liu SH Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway. Infect. Immun., 2009-05-26;77(8):3312-9. 2009-05-26 [PMID: 19470750] (Neutralization, Mouse)	Neutralization	Mouse
Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH Foxa1 and Foxa2 regulate bile duct development in mice. J. Clin. Invest., 2009-05-11;119(6):1537-45. 2009-05-11 [PMID: 19436110] (IHC-Fr, Neutralization, Mouse)	IHC-Fr, Neutralization	Mouse
Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice. Eur. J. Pharmacol., 2008-07-10;592(1):87-92. 2008-07-10 [PMID: 18652822] (Neutralization, Mouse)	Neutralization	Mouse
Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-05-09;295(1):L79-85. 2008-05-09 [PMID: 18469117] (IHC, Rabbit)	IHC	Rabbit
Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo. J. Immunol., 2008-03-15;180(6):4227-34. 2008-03-15 [PMID: 18322235] (Neutralization, Mouse)	Neutralization	Mouse
Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones. Mol. Cell. Neurosci., 2007-09-11;37(1):110-8. 2007-09-11 [PMID: 17933551] (ICC, Mouse)	ICC	Mouse
Meagher MW, Johnson RR, Young EE, Vichaya EG, Lunt S, Hardin EA, Connor MA, Welsh CJ Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theiler's virus infection. Brain Behav. Immun., 2007-06-25;21(8):1083-95. 2007-06-25 [PMID: 17591434] (Neutralization, Mouse)	Neutralization	Mouse
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (IHC, Mouse)	IHC	Mouse
Kurooka M, Kaneda Y Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res., 2007-01-01;67(1):227-36. 2007-01-01 [PMID: 17210703] (Neutralization, Mouse)	Neutralization	Mouse
Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors. J. Invest. Dermatol., 2005-11-01;125(5):1053-62. 2005-11-01 [PMID: 16297209] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Arch. Dermatol. Res., 2001-11-01;293(10):508-14. 2001-11-01 [PMID: 11820727] (Neutralization, Mouse)	Neutralization	Mouse
Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (Neutralization, Mouse)	Neutralization	Mouse
Show All 48 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-Fr	Mouse	01/08/2015	View Summary Application Immunohistochemistry-Frozen Sample Tested See PMID 20683902 Species Mouse

Array AF-406-NA

• IL-6 Antibodies
• IL-6 Antibody Pair
• IL-6 ELISA Kits
• IL-6 Lysate
• IL-6 Proteins
• IL-6 ELISAs
• IL-6 Proteins and Enzymes