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IL-21 Antibody - BSA Free

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Expression of Tagged Proteins: Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 ...read more
Immunocytochemistry/ Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. ...read more
Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% ...read more
Independent Antibodies: Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 ...read more
Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human HL-60 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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IL-21 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21. Amino Acid Squence: TCPSCDSYEKKPPKE
Specificity
At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL21
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2 ug/mL
  • Western Blot 1 ug/mL
Application Notes
Use in B/N reported in scientific literature (PMID:34408754).
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IL-21 Peptide (NBP1-76740PEP)
Publications
Read Publication using
NBP1-76740 in the following applications:

  • B/N
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Pig: (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for IL-21 Antibody - BSA Free

  • CVID11
  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform
  • Za11

Background

A novel cytokine was recently identified in human and mouse and designated IL-21 , which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-21 Antibody (NBP1-76740)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-21 Antibody (NBP1-76740) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IL-21 Products

Research Areas for IL-21 Antibody (NBP1-76740)

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Blogs on IL-21.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol IL21
Uniprot