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IL-21 Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF594
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF594
Carrier Free
AF594
Carrier Free
AF594-SP
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IL-21 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL‑15, and recombinant human IL‑21 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL21
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse IL-21 Antibody (Catalog # AF594)
ELISA Detection: Mouse IL-21 Biotinylated Antibody (Catalog # BAF594)
Standard: Recombinant Mouse IL-21 (Catalog # 594-ML)
Readout System
Publications
Read Publications using
BAF594 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21 Antibody [Biotin]

  • CVID11
  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform
  • Za11

Background

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha . Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-21 Antibody
[Biotin]
BAF594
IL-21 Antibody [Biotin]
Species: Mu
Applications: WB, ELISA(Det)

Publications for IL-21 Antibody (BAF594)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Detection, ELISA Development, ELISpot Development, Flow Cytometry.


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ELISA Detection
(1)
ELISA Development
(3)
ELISpot Development
(1)
Flow Cytometry
(2)
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Mouse
(7)
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Showing Publications 1 - 8 of 8.
Publications using BAF594 Applications Species
Akgul, A;Freguia, CF;Maddaloni, M;Hoffman, C;Voigt, A;Nguyen, CQ;Fanger, NA;Fanger, GR;Pascual, DW; Treatment with a Lactococcus lactis that chromosomally express E. coli cfaI mitigates salivary flow loss in a Sjögren's syndrome-like disease Scientific reports 2023-11-09 [PMID: 37945636] (ELISA Detection, Mouse) ELISA Detection Mouse
KM Hooper, W Kong, D Ganea Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf PLoS ONE, 2017-06-12;12(6):e0179184. 2017-06-12 [PMID: 28604806] (Mouse) Mouse
Eliana Mariño, Bernice Tan, Lauren Binge, Charles R. Mackay, Shane T. Grey B-Cell Cross-Presentation of Autologous Antigen Precipitates Diabetes Diabetes 2012-11-01 [PMID: 22829452]
McGuire HM, Walters S, Vogelzang A Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models. Diabetes, 2011-03-01;60(3):867-75. 2011-03-01 [PMID: 21357471] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Chen G, Hardy K, Bunting K Regulation of the IL-21 gene by the NF-kappaB transcription factor c-Rel. J. Immunol., 2010-07-16;185(4):2350-9. 2010-07-16 [PMID: 20639489] (ELISA Development, Flow Cytometry, Mouse) ELISA Development, Flow Cytometry Mouse
Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE). PLoS ONE, 2010-01-14;5(1):e8720. 2010-01-14 [PMID: 20090936] (ELISA Development, Mouse) ELISA Development Mouse
Reinhardt RL, Liang HE, Locksley RM Cytokine-secreting follicular T cells shape the antibody repertoire. Nat. Immunol., 2009-03-01;10(4):385-93. 2009-03-01 [PMID: 19252490] (ELISpot Development, Mouse) ELISpot Development Mouse
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol IL21
Uniprot