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IL-17RA/IL-17R Antibody

 (8 Publications)
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RA/IL-17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL‑6 Quantikine ELISA Kit (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF177
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF177-SP
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IL-17RA/IL-17R Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Leu33-Trp320
Accession # Q96F46
Specificity
Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL‑17 RA/IL‑17 R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17RA
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.1-0.5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF177 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RA/IL-17R Antibody

  • CD217 antigen
  • CD217
  • Cdw217
  • CDw217interleukin 17 receptor
  • hIL-17R
  • IL-17 R
  • IL-17 RA
  • IL-17 receptor A
  • IL17RA
  • IL-17RA
  • IL-17RAMGC10262
  • IL17Rinterleukin-17 receptor A
  • interleukin 17 receptor A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17RA/IL-17R Antibody (AF177)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, IHC, Neutralization, Western Blot.


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Flow Cytometry
(1)
IHC
(1)
Neutralization
(2)
Western Blot
(1)
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Human
(6)
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Showing Publications 1 - 8 of 8.
Publications using AF177 Applications Species
D Koslawsky, M Zaretsky, R Alcalay, O Mazor, A Aharoni, N Papo A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells Oncotarget, 2018-06-19;9(47):28500-28513. 2018-06-19 [PMID: 29983876] (Human) Human
Liu , Xiaosun, Jin , Hailong, Zhang , Geer, Lin , Xianke, Chen , Chao, Sun , Jianyi, Zhang , Yu, Zhang , Qing, Yu , Jiren Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients. PLoS ONE, 2014-09-08;9(9):e106834. 2014-09-08 [PMID: 25197971] (IHC, Human) IHC Human
Taiji Nakashima, Masatoshi Jinnin, Keitaro Yamane, Noritoshi Honda, Ikko Kajihara, Takamitsu Makino et al. Impaired IL-17 Signaling Pathway Contributes to the Increased Collagen Expression in Scleroderma Fibroblasts The Journal of Immunology 4/15/2012 [PMID: 22403442]
Lucie Roussel, François Houle, Carlos Chan, Yu Yao, Julie Bérubé, Ron Olivenstein et al. IL-17 Promotes p38 MAPK-Dependent Endothelial Activation Enhancing Neutrophil Recruitment to Sites of Inflammation The Journal of Immunology 4/15/2010 [PMID: 20228195]
Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin&#039;s lymphoma. Int. J. Cancer, 2009-09-01;125(5):1092-101. 2009-09-01 [PMID: 19391137] (Flow Cytometry, Human) Flow Cytometry Human
Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 2008-09-15;181(6):4299-310. 2008-09-15 [PMID: 18768888] (Neutralization, Human) Neutralization Human
Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J. Immunol., 2008-08-15;181(4):2799-805. 2008-08-15 [PMID: 18684971] (Western Blot, Human) Western Blot Human
Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 2004-09-01;173(5):3482-91. 2004-09-01 [PMID: 15322213] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol IL17RA
Uniprot