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IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

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Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications Flow, ICC/IF
Clone
1B1.3a
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08221AF350
Alexa Fluor 405
NBP3-08221AF405
Alexa Fluor 488
NBP3-08221AF488
Alexa Fluor 532
NBP3-08221AF532
Alexa Fluor 594
NBP3-08221AF594
Alexa Fluor 647
NBP3-08221AF647
Alexa Fluor 700
NBP3-08221AF700
Alexa Fluor 750
NBP3-08221AF750
Allophycocyanin
NBP3-08221APC
Biotin
NBP3-08221B
CoraFluor 1
NBP3-08221CL1
DyLight 350
NBP3-08221UV
DyLight 405
NBP3-08221V
DyLight 488
NBP3-08221G
DyLight 550
NBP3-08221R
DyLight 594
NBP3-08221DL594
DyLight 650
NBP3-08221C
DyLight 680
NBP3-08221FR
DyLight 755
NBP3-08221IR
FITC
NBP3-08221F
HRP
NBP3-08221H
Janelia Fluor 525
NBP3-08221JF525
Janelia Fluor 549
NBP3-08221JF549
Janelia Fluor 585
NBP3-08221JF585
Janelia Fluor 635
NBP3-08221JF635
Janelia Fluor 646
NBP3-08221JF646
Janelia Fluor 669
NBP3-08221JF669
PE
NBP3-08221PE
PerCP
NBP3-08221PCP
Unconjugated
NBP3-07330-100ug
mFluor Violet 450 SE
NBP3-08221MFV450
mFluor Violet 500 SE
NBP3-08221MFV500
mFluor Violet 610 SE
NBP3-08221MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07330-100ug
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IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07330).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Purified recombinant extracellular region of Mouse IL-10R alpha (Uniprot: Q61727)
Localization
Cell Surface
Specificity
This monoclonal antibody recognizes Mouse Interleukin-10 receptor 1 (IL-10R1), a cell surface glycoprotein of approximately 110KDa. Its epitope is localized in the ligand-binding domain in the extracellular region of Mouse IL-10R1. This antibody blocks the binding of IL-10 to its receptor. IL-10R1 is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Binding of IL-10 to its receptor initiates JAK/STAT signaling that leads to B cell proliferation and inhibition of macrophage activation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
IL10RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
Application Notes
Neutralization Studies: Order antibody without Azide

Reactivity Notes

Does not react with Human.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-10R alpha Antibody (1B1.3a) - Azide and BSA Free

  • CD210 antigen
  • CDw210a
  • HIL-10R
  • IBD28
  • IL-10 R alpha
  • IL-10 receptor subunit alpha
  • IL10R alpha
  • IL-10R subunit 1
  • IL-10R1
  • IL10RA
  • IL-10Ra
  • IL10RIL-10R subunit alpha
  • interleukin 10 receptor, alpha
  • interleukin-10 receptor alpha chain
  • Interleukin-10 receptor subunit 1
  • interleukin-10 receptor subunit alpha

Background

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IL10RA