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IL-10 R beta Antibody (90220) [Phycoerythrin]

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Human PBMC monocytes were stained with Mouse Anti-Human IL-10 R beta PE-conjugated Monoclonal Antibody (Catalog # FAB874P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
90220
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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IL-10 R beta Antibody (90220) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334
Specificity
Detects human IL-10 R beta  in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑10 R alpha , rhIFN-gamma  RI, and rhIFN-gamma  R2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL10RB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB874P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 R beta Antibody (90220) [Phycoerythrin]

  • CDw210b antigen
  • CDw210b
  • CRF2-4IL-10R subunit 2
  • CRFB4
  • CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
  • D21S58
  • D21S66
  • IBD25
  • IL-10 R beta
  • IL-10 receptor subunit beta
  • IL10R beta
  • IL-10R2
  • IL-10R2IL-10R subunit beta
  • IL10RB
  • IL-10Rb
  • interleukin 10 receptor, beta
  • Interleukin-10 receptor subunit 2
  • interleukin-10 receptor subunit beta
  • member 4

Background

IL-10 R beta , also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
  3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
  5. Lutfalla, G. et al. (1993) Genomics 16:366.
  6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 R beta Antibody (FAB874P)(3)

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Bioinformatics

Gene Symbol IL10RB
Uniprot