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IL-1 alpha/IL-1F1 Antibody (40508) [Unconjugated]

 (8 Publications)
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Recombinant MouseIL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB400). The ND50 is typically 0.2-0.6 µg/mL.
Recombinant MouseIL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
40508
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB400-SP
Carrier Free
MAB400-500
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IL-1 alpha/IL-1F1 Antibody (40508) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL-1 alpha /IL-1F1
Ser115-Ser270
Accession # P01582
Specificity
Detects mouse IL-1 alpha /IL-1F1 in ELISAs. In ELISAs, less than 1% cross-reactivity with recombinant rat IL‑1 alpha /IL‑1F1, recombinant porcine
IL‑1 alpha /IL‑1F1, and recombinant human IL‑1 alpha /IL‑1F1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
IL1A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.2-0.6 ug/mL
Application Notes
ELISA Detection: Mouse IL-1alpha /IL-1F1 Biotinylated Antibody (Catalog number BAF400)
Standard: Recombinant Mouse IL-1alpha /IL-1F1 (Catalog number 400-ML)
Publications
Read Publications using
MAB400 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody (40508) [Unconjugated]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta , that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta , designated interleukin 1 beta ‑converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4, and IL-13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-1 alpha/IL-1F1 Antibody (40508) [Unconjugated]
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Publications for IL-1 alpha/IL-1F1 Antibody (MAB400)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, ELISA Development, ELISA Development (Capture), Neutralization.


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Bioassay
(1)
ELISA Development
(2)
ELISA Development (Capture)
(1)
Neutralization
(4)
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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using MAB400 Applications Species
A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain Nat. Cell Biol., 2019-05-13;0(0):. 2019-05-13 [PMID: 31086261] (Bioassay, Mouse) Bioassay Mouse
Alternatively activated macrophages determine repair of the infarcted adult murine heart J Clin Invest, 2016-05-03;0(0):. 2016-05-03 [PMID: 27140396] (Neutralization, Mouse) Neutralization Mouse
Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Nat Med, 2015-08-03;21(9):1085-90. 2015-08-03 [PMID: 26236992] (Neutralization, Mouse) Neutralization Mouse
Fu X, Xiao J, Wei Y, Li S, Liu Y, Yin J, Sun K, Sun H, Wang H, Zhang Z, Zhang B, Sheng C, Wang H, Hu P Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion. Cell Res, 2015-05-15;25(6):655-73. 2015-05-15 [PMID: 25976405] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. J Invest Dermatol, 2013-11-25;134(5):1295-304. 2013-11-25 [PMID: 24352037] (Neutralization, Mouse) Neutralization Mouse
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Mouse) ELISA Development Mouse
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009-07-10;0(0):. 2009-07-10 [PMID: 19592492] (ELISA Development, Mouse) ELISA Development Mouse
Bitko V, Musiyenko A, Barik S Viral infection of the lungs through the eye. J. Virol., 2006-10-18;81(2):783-90. 2006-10-18 [PMID: 17050596] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol IL1A
Uniprot