Reactivity | ChSpecies Glossary |
Applications | WB, ELISA, IHC |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgM and Chicken Serum |
Immunogen | Chicken IgM whole molecule |
Specificity | No reaction was observed against other chicken heavy or light chain proteins. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | IGHM |
Purity | Multi-step |
Dilutions |
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Application Notes | This product has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product. |
Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% Sodium Azide |
Concentration | LYOPH |
Purity | Multi-step |
Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
Research Areas for IgM Heavy Chain Antibody (NBP1-73506)Find related products by research area.
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Gene Symbol | IGHM |