![Western Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73572] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-F-ab-2-anti-Chicken-IgG-Secondary-Antibody-HRP-Western-Blot-NBP1-73572-img0004.jpg "Western Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [HRP] [NBP1-73572] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.")
| Reactivity | ChSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72631 | |||
| Biotin | NBP1-73508 | |||
| FITC | NBP1-73280 | |||
| Rhodamine | NBP1-73278 | |||
| Texas Red | NBP1-73509 | |||
| Unconjugated | NBP1-72800 | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum |
| Immunogen | Chicken IgG F(ab')2 fragment |
| Specificity | No reaction was observed against Chicken IgG F(c). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Dilutions |
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| Application Notes | This product has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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