![Western Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Texas Red] [NBP1-73509] - Western Blot of Rabbit anti-Chicken IgG F(ab')2 Secondary antibody [Texas Red]. Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Chicken-IgG-F-ab-2-Secondary-Antibody-Texas-Red-Western-Blot-NBP1-73509-img0001.jpg "Western Blot: Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody [Texas Red] [NBP1-73509] - Western Blot of Rabbit anti-Chicken IgG F(ab')2 Secondary antibody [Texas Red]. Lane M: 3 ul Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.")
| Reactivity | ChSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Texas Red |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-72631 | |||
| Biotin | NBP1-73508 | |||
| FITC | NBP1-73280 | |||
| HRP | NBP1-73572 | |||
| Rhodamine | NBP1-73278 | |||
| Unconjugated | NBP1-72800 | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2, and Chicken Serum |
| Immunogen | Chicken IgG F(ab')2 fragment |
| Specificity | No reaction was observed against Chicken IgG F(c). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Dilutions |
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| Application Notes | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with 1.0 ml deionized water (or equivalent). |
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