![Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) [NBP1-51902] - Human cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Human-IgG-H+L-Secondary-Antibody-DyLight-649-Pre-adsorbed-Flow-Cytometry-NBP1-51902-img0001.jpg "Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) [NBP1-51902] - Human cancer cell line. Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | DyLight 649 |
| Format | Pre-adsorbed |
| Concentration | 0.5 mg/ml |
| Immunogen | Human IgG-F(ab')2 Fragment |
| Specificity | Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
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| Reviewed Applications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS containing 0.2% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Flow | Human | 09/24/2018 |
Summary
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