![Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900] - Human cancer cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-F-ab-2-anti-Human-IgG-F-ab-2-Secondary-Antibody-Pre-adsorbed-Flow-Cytometry-NBP1-51900-img0001.jpg "Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody (Pre-adsorbed) [NBP1-51900] - Human cancer cell line. Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | Pre-adsorbed |
| Concentration | 0.5 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 649 | NBP1-51902 | |||
| Unconjugated | NBP1-51902 | |||
| Immunogen | Human IgG-F(ab')2 Fragment |
| Specificity | By immunoelectrophoresis and ELISA this antibody reacts specifically with F(ab')2 fragments of human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 fragments of IgG from other species. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Dilutions |
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| Application Notes | Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. ELISA, for coating plates 1:50 - 1:250. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate Buffered Saline (PBS) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-51900 | Applications | Species |
|---|---|---|
| Zhou Y, Wen P, Li M et al. Construction of chimeric antigen receptor modified T cells targeting EpCAM and assessment of their anti tumor effect on cancer cells Mol Med Rep 2019-09-01 [PMID: 31322180] |
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