Home » IGFBP-rp1/IGFBP-7 » IGFBP-rp1/IGFBP-7 Antibodies » IGFBP-rp1/IGFBP-7 Antibody (192520) [Unconjugated]
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IGFBP-rp1/IGFBP-7 Antibody (192520) [Unconjugated]

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Recombinant Human IGFBP‑rp1/IGFBP‑7 protein was serially diluted 2-fold and captured by Mouse Anti-Human IGFBP‑rp1/IGFBP‑7 Monoclonal Antibody (Catalog # MAB13342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IGFBP‑rp1/IGFBP‑7 Monoclonal Antibody (Catalog # MAB1334) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human IGFBP‑rp1/IGFBP‑7 protein was serially diluted 2-fold and captured by Mouse Anti-Human IGFBP‑rp1/IGFBP‑7 Monoclonal Antibody (Catalog # MAB13342) coated on a Clear Polystyrene ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
192520
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1334-SP
Carrier Free
MAB1334-500
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IGFBP-rp1/IGFBP-7 Antibody (192520) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1/IGFBP-7
Asp30-Arg277
Accession # AAA16187
Specificity
Detects human IGFBP‑rp1/IGFBP-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, or -6 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGFBP7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1334 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-rp1/IGFBP-7 Antibody (192520) [Unconjugated]

  • angiomodulin
  • FSTL2
  • IBP-7
  • IGFBP7
  • IGFBP-7
  • IGFBP-7PGI2-stimulating factor
  • IGFBP-7v
  • IGFBPrp1
  • IGFBP-rp1
  • insulin-like growth factor binding protein 7
  • insulin-like growth factor-binding protein 7
  • MAC25 protein
  • Mac25
  • MAC25IGF-binding protein 7
  • Prostacyclin-stimulating factor
  • PSF
  • PSFAGM
  • TAF
  • Tumor-derived adhesion factor

Background

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Mature
IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal
immunoglobulin‑like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGFBP-rp1/IGFBP-7 Antibody (MAB1334)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, Western Blot.


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ELISA Development
(1)
Western Blot
(2)
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(2)
Mouse
(1)
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Showing Publications 1 - 6 of 6.
Publications using MAB1334 Applications Species
Juan Gao, Shiqi Suo, Jingxia Li, Chunxia Wang, Ranran Deng, Yuxia Hu et al. IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway Experimental and Therapeutic Medicine 3/3/2023 [PMID: 37006880]
HJMP Verhagen, N van Gils, T Martiañez, A van Rhenen, A Rutten, F Denkers, DC de Leeuw, MA Smit, ML Tsui, LLE de Vos Klo, RX Menezes, M Çil, MGM Roemer, E Vermue, S Heukelom, S Zweegman, JJWM Janssen, GJ Ossenkoppe, GJ Schuurhuis, L Smit IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis Cell Rep, 2018-12-11;25(11):3021-3035.e5. 2018-12-11 [PMID: 30540936] (Western Blot, Mouse) Western Blot Mouse
Yu Ma, Jue Jiang, Yan Zhang, Yi Ding, Ting Xu, Bingjian Lu IGFBP-rP1 acts as a potential tumor suppressor via the suppression of ERK signaling pathway in endometrial cancer cells Molecular Medicine Reports 2/1/2017 [PMID: 29067463]
H JMP Verhagen, D C de Leeuw, M GM Roemer, F Denkers, W Pouwels, A Rutten et al. IGFBP7 induces apoptosis of acute myeloid leukemia cells and synergizes with chemotherapy in suppression of leukemia cell survival Cell Death & Disease 6/26/2014 [PMID: 24967962]
Gunawardana CG, Kuk C, Smith CR Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer. J. Proteome Res., 2009-10-01;8(10):4705-13. 2009-10-01 [PMID: 19663500] (ELISA Development, Human) ELISA Development Human
Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol. Cell Proteomics, 2005-10-08;5(1):157-71. 2005-10-08 [PMID: 16215274] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol IGFBP7
Uniprot