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IFT74 Antibody

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Western Blot: IFT74 Antibody [NB300-822] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antinbody at 0.2 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IFT74 Antibody Summary

Immunogen
Peptide with sequence C-KTIVDALHSTSGN corresponding to C-Terminus according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFT74
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 0.6 ug/mL
Application Notes
IF successful usage for stained rat cortical neurons is reported in scientific literature (PMID: 17166276). WB: Approx. 70-75 kDa band observed in HEK293 lysates is reported in scientific literature (PMID: 17166276).
Control
293 Whole Cell Lysate
Publications
Read Publications using NB300-822.

Reactivity Notes

Expected species reactivity based on sequence similarity: Rat

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFT74 Antibody

  • Capillary morphogenesis gene 1 protein
  • CCDC2capillary morphogenesis protein 1
  • CMG1Coiled-coil domain-containing protein 2
  • CMG-1MGC111562
  • coiled-coil domain containing 2
  • FLJ22621
  • intraflagellar transport 74 homolog (Chlamydomonas)
  • intraflagellar transport protein 74 homolog

Background

The IFT74 gene codes for an intraflagellar transport protein 74 homolog. Isoform 1 is 600 amino acids long at 69 kDA while isoform 2 exists at 409 amino acids long at 46 kDA. IFT74 has been linked to dementia, lateral sclerosis, neuronitis, amyotrphic lateral sclerosis, and frontotemporal dementia. It is known to interact with other genes such as: IFT27, IFT52, IFT81, IFT88, and DYNC2LI1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFT74 Antibody (NB300-822)(2)

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Bioinformatics

Gene Symbol IFT74
Entrez
Uniprot